Multi-organ histological assessment of Agrococcus sp. RKDAS_1 reveals tissue-level biocompatibility in Oreochromis niloticus

IF 2.2 4区生物学 Q3 CELL BIOLOGY

Journal of Molecular Histology Pub Date : 2026-05-04 DOI:10.1007/s10735-026-10819-x

Venkatesan Karthick, Arun Venkatesh, Singamoorthy Amalraj, Sulochana Sonti, Rajkumar Thamarai, Rajkumar Prabhakaran

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引用次数: 0

Abstract

A histological safety profile is essential for non-antibiotic microbial interventions in aquaculture. This study assessed the multi-organ histological biocompatibility of a novel actinobacterial strain, Agrococcus sp. RKDAS_1, in Nile tilapia (Oreochromis niloticus) under controlled conditions. Juvenile tilapia were fed diets with varying concentrations of RKDAS_1 (10⁵, 10⁷, and 10⁹ CFU g^{− 1} feed) for 60 days. Tissues from the gill, intestine, liver, and heart were analysed through standard histological methods, employing semiquantitative scoring and intestinal morphometry. Across all examined organs, RKDAS_1 supplementation did not induce inflammatory responses, degenerative lesions, or structural disruptions, which are indicative of tissue-level toxicity. Gill architecture was intact, with normal hepatocyte arrangements and no necrosis or fibrosis, while cardiac tissues showed a normal structure. Intestinal morphology maintained epithelial integrity, displaying dose-related variations in villus height and goblet cell density. Intermediate-dose live hepatic sections showed reduced cytoplasmic vacuolation compared to controls, though the difference was not statistically significant. The semi-quantitative histopathological evaluation showed that the tissue structure remained intact across different treatments. The results collectively suggest that Agrococcus sp. RKDAS_1 did not cause any noticeable histopathological damage, indicating enhanced biocompatibility. The findings suggest that Agrococcus sp. RKDAS_1 is compatible with biological tissues without causing significant damage. However, these conclusions are restricted to structural analysis and do not ensure functional improvements or overall safety beyond tissue examination. The findings provide a critical starting point for future research, which will delve into molecular, immunological, and long-term exposure studies. These investigations aim to evaluate the biotherapeutic potential of RKDAS_1 thoroughly.

Graphical abstract

The alternative text for this image may have been generated using AI.

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农球菌RKDAS_1的多器官组织学评价揭示了其在尼罗褐虾中的组织水平生物相容性。

组织学安全概况是必不可少的非抗生素微生物干预水产养殖。研究了一种新型放线菌Agrococcus sp. RKDAS_1在尼罗罗非鱼（Oreochromis niloticus）体内的多器官组织生物相容性。分别饲喂不同浓度RKDAS_1（105、107和109 CFU g- 1饲料）的罗非鱼幼鱼60 d。通过标准组织学方法分析鳃、肠、肝和心脏组织，采用半定量评分和肠形态计量学。在所有被检测的器官中，RKDAS_1的补充没有引起炎症反应、退行性病变或结构破坏，这些都表明了组织水平的毒性。鳃结构完整，肝细胞排列正常，无坏死或纤维化，心脏组织结构正常。肠道形态保持上皮完整，绒毛高度和杯状细胞密度呈现剂量相关变化。与对照组相比，中剂量活肝切片显示胞浆空泡化减少，但差异无统计学意义。半定量组织病理学评估显示，不同处理的组织结构保持完整。综上所述，Agrococcus sp. RKDAS_1未引起明显的组织病理学损伤，表明其生物相容性增强。结果表明，农球菌RKDAS_1与生物组织具有相容性，且无明显损伤。然而，这些结论仅限于结构分析，并不能确保在组织检查之外的功能改善或整体安全性。这些发现为未来的研究提供了一个关键的起点，未来的研究将深入到分子、免疫学和长期暴露研究中。这些研究旨在全面评估RKDAS_1的生物治疗潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Molecular Histology 生物-细胞生物学

CiteScore

5.90

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： The Journal of Molecular Histology publishes results of original research on the localization and expression of molecules in animal cells, tissues and organs. Coverage includes studies describing novel cellular or ultrastructural distributions of molecules which provide insight into biochemical or physiological function, development, histologic structure and disease processes. Major research themes of particular interest include: - Cell-Cell and Cell-Matrix Interactions; - Connective Tissues; - Development and Disease; - Neuroscience. Please note that the Journal of Molecular Histology does not consider manuscripts dealing with the application of immunological or other probes on non-standard laboratory animal models unless the results are clearly of significant and general biological importance. The Journal of Molecular Histology publishes full-length original research papers, review articles, short communications and letters to the editors. All manuscripts are typically reviewed by two independent referees. The Journal of Molecular Histology is a continuation of The Histochemical Journal.