High-Throughput Sequencing Reveals Previously Undetected Viruses and Mixed Infections in Pepper (Capsicum annuum) in Hungary.

IF 3.5 3区医学 Q2 VIROLOGY

Viruses-Basel Pub Date : 2026-04-21 DOI:10.3390/v18040481

Emese Demián, Réka Sáray, Asztéria Almási, Kata Pogácsás, Katalin Salánki

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引用次数: 0

Abstract

The increasing global movement of plant material and the complexity of viral communities associated with cultivated crops complicate routine plant virus diagnostics. High-throughput sequencing (HTS) has therefore become an important tool for the comprehensive characterization of plant viromes. In this study, symptomatic pepper (Capsicum annuum) samples submitted to our laboratory between 2020 and 2025 were investigated using HTS following unsuccessful routine diagnostic assays, despite the presence of virus-like symptoms. Virome analysis revealed the presence of multiple viruses with distinct biological characteristics. Eggplant mottled dwarf virus (EMDV) sequences were identified, representing, to our knowledge, the first sequence data from Hungary. In addition, sequences related to tobacco vein clearing virus (TVCV) showed highest similarity to endogenous viral element present in Capsicum annuum genome assemblies. Persistent viruses, including bell pepper alphaendornavirus (BPEV) and pepper cryptic virus 2 (PCV2), were also detected. These findings demonstrate the complex viral communities associated with cultivated pepper and highlight the limitations of strictly targeted diagnostic approaches. The results emphasize the value of HTS for comprehensive virome characterization in horticultural crops.

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高通量测序揭示了匈牙利辣椒（辣椒）中以前未检测到的病毒和混合感染。

日益增加的全球植物材料流动和与栽培作物相关的病毒群落的复杂性使常规的植物病毒诊断复杂化。因此，高通量测序（HTS）已成为植物病毒组综合鉴定的重要工具。在这项研究中，尽管存在病毒样症状，但在2020年至2025年期间提交给我们实验室的有症状辣椒（Capsicum annuum）样本在常规诊断分析失败后，使用HTS进行了调查。病毒组分析显示存在多种具有不同生物学特性的病毒。茄子斑驳矮病毒（EMDV）序列被鉴定出来，据我们所知，这是来自匈牙利的第一个序列数据。此外，烟草清脉病毒（TVCV）相关序列与辣椒基因组中存在的内源病毒元件的相似性最高。辣椒α内啡肽病毒（BPEV）和辣椒隐病毒2 （PCV2）等持续性病毒也被检测到。这些发现证明了与栽培辣椒相关的复杂病毒群落，并突出了严格定向诊断方法的局限性。结果强调了HTS技术在园艺作物病毒组综合鉴定中的价值。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Viruses-Basel VIROLOGY-

CiteScore

7.30

自引率

12.80%

发文量

2445

审稿时长

1 months

期刊介绍： Viruses (ISSN 1999-4915) is an open access journal which provides an advanced forum for studies of viruses. It publishes reviews, regular research papers, communications, conference reports and short notes. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. We also encourage the publication of timely reviews and commentaries on topics of interest to the virology community and feature highlights from the virology literature in the ''News and Views'' section. Electronic files or software regarding the full details of the calculation and experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material.