The Laccase-like Property of GHK-Cu and Its Applications in Colorimetric Sensing of Phenolic Compounds.

IF 5.6 3区工程技术 Q1 CHEMISTRY, ANALYTICAL

Biosensors-Basel Pub Date : 2026-04-12 DOI:10.3390/bios16040217

Jiang-Shan Chen, Huan Zhu, Tong-Qing Chai, Feng-Qing Yang

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引用次数: 0

Abstract

Laccase plays an important role in the detection and degradation of phenolic compounds, but it is limited by its cost and stability. In this study, the laccase-like property of copper peptide (GHK-Cu) has been revealed. In terms of enzymatic reaction kinetics, GHK-Cu has a V_max of 1.735 × 10^-4 mM·s^-1 and a K_m of 0.061 mM, demonstrating good substrate affinity and excellent catalytic efficiency. Then, a colorimetry was developed for rapid detection of epinephrine (EP) and 2-aminophenol (2-AP). The linear response range of EP is 20-240 μM, with a limit of detection (LOD) of 9.5 μM. The linear response ranges of 2-AP are 14-100 μM (in ultrapure water) and 2-120 μM (in seawater), with LODs of 2.56 μM and 1.65 μM. In addition, combined with a smartphone platform, a cotton-based sensor has been developed for the detection of 2-AP in seawater. The linear response ranges are 0-0.2 mM and 0.2-1 mM, with LOD of 0.033 mM. The structure of GHK-Cu provides a reference for the development of novel laccase mimetic enzymes. The constructed colorimetry offers an option for the rapid detection of phenolic compounds, and the developed cotton-based sensor enabled rapid and portable detection of 2-AP.

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GHK-Cu漆酶样性质及其在酚类化合物比色检测中的应用。

漆酶在检测和降解酚类化合物方面发挥着重要作用，但其成本和稳定性限制了漆酶的应用。本研究揭示了铜肽（GHK-Cu）的漆酶样性质。在酶促反应动力学方面，GHK-Cu的Vmax为1.735 × 10-4 mM·s-1， Km为0.061 mM，表现出良好的底物亲和性和催化效率。然后，建立了快速检测肾上腺素（EP）和2-氨基酚（2-AP）的比色法。电位线性响应范围为20 ~ 240 μM，检出限为9.5 μM。2-AP的线性响应范围为14 ~ 100 μM（超纯水）和2 ~ 120 μM（海水），lod分别为2.56 μM和1.65 μM。此外，结合智能手机平台，开发了用于检测海水中2-AP的棉花传感器。线性响应范围为0 ~ 0.2 mM和0.2 ~ 1 mM， LOD为0.033 mM。GHK-Cu的结构为开发新型漆酶模拟酶提供了参考。构建的比色法为酚类化合物的快速检测提供了一种选择，开发的基于棉花的传感器可以快速和便携式地检测2-AP。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biosensors-Basel Biochemistry, Genetics and Molecular Biology-Clinical Biochemistry

CiteScore

6.60

自引率

14.80%

发文量

983

审稿时长

11 weeks

期刊介绍： Biosensors (ISSN 2079-6374) provides an advanced forum for studies related to the science and technology of biosensors and biosensing. It publishes original research papers, comprehensive reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.