Origins of Atlantic Salmon (Salmo salar) Determined Using a Hybridization Assay of Mitochondrial DNA on a Microfluidic Biochip.

IF 5.6 3区工程技术 Q1 CHEMISTRY, ANALYTICAL

Biosensors-Basel Pub Date : 2026-04-21 DOI:10.3390/bios16040231

Lin Wang, Christopher Oberc, Krzysztof P Lubieniecki, William S Davidson, Paul C H Li

引用次数: 0

Abstract

A hybridization assay based on the microfluidic biochip was developed to identify the origin of the Atlantic salmon species. Among the 215 single nucleotide polymorphic (SNP) sites found in the mtDNA of Salmo salar, we located five sites in devising our assay method. We found two sites that worked, while the others generated either insufficient signals or specificity. We have successfully identified the North American origin of the three samples, as confirmed by Sanger sequencing.

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用微流控生物芯片上的线粒体DNA杂交测定大西洋鲑鱼（Salmo salar）的起源。

基于微流控生物芯片的杂交试验被开发用于鉴定大西洋鲑鱼物种的起源。在Salmo salar的mtDNA中发现的215个单核苷酸多态性（SNP）位点中，我们在设计检测方法时定位了5个位点。我们发现了两个有效的位点，而其他的位点要么产生了不足的信号，要么产生了特异性。经桑格测序证实，我们已成功鉴定出这三个样本的北美来源。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biosensors-Basel Biochemistry, Genetics and Molecular Biology-Clinical Biochemistry

CiteScore

6.60

自引率

14.80%

发文量

983

审稿时长

11 weeks

期刊介绍： Biosensors (ISSN 2079-6374) provides an advanced forum for studies related to the science and technology of biosensors and biosensing. It publishes original research papers, comprehensive reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.