miR-1247-5p is correlated with cervical cancer and regulates cervical cancer cell functions by targeting DVL1.

Abstract

The incidence of cervical cancer (CC) is extremely high, yet current diagnostic biomarkers have not achieved satisfactory results. MicroRNAs can regulate vital processes in tumors, including proliferation and apoptosis. The regulatory role of miR-1247-5p in CC remains unknown. This study aims to investigate the diagnostic value of miR-1247-5p in CC. This study included 156 patients with CC and 130 healthy volunteers. Real-time quantitative PCR was used to detect miR-1247-5p and Dishevelled 1 (DVL1) levels. Cell proliferation was assessed using the CCK-8 assay. Levels of Fe2+, lipid reactive oxygen species, malondialdehyde, and glutathione were measured using commercial kits. Diagnostic value of each biomarker was evaluated via receiver operating characteristic curve analysis. miR-1247-5p is significantly downregulated in CC. Low miR-1247-5p constitutes one of the risk factors for CC development. miR-1247-5p mimic inhibits proliferation and promotes ferroptosis. DVL1 is a target of miR-1247-5p. It is upregulated in both serum from CC patients and CC cells. oe-DVL1 partially reverses the suppression of CC malignant behavior by miR-1247-5p mimic. Therefore, we conclude that miR-1247-5p suppresses the malignant behavior of CC by targeting DVL1 and may serve as a potential clinical diagnostic biomarker.