Integrated Genomic and Phenotypic Analyses Reveal Convergent Resistance Patterns in Clinical Candida tropicalis Isolates.

IF 3.1 2区医学 Q1 DERMATOLOGY

Mycoses Pub Date : 2026-05-01 DOI:10.1111/myc.70181

Christy Chedraoui, Nour Fattouh, Setrida El Hachem, Tsolaire Sourenian, Louna El Bitar, Lama El Moheb, Anna-Souraya Sleiman, Ibrahim Bitar, Rola Husni, Roy A Khalaf

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引用次数: 0

Abstract

Background: The incidence of Candida tropicalis isolation is increasing in hospital settings. High azole resistance and mortality rates make it a pathogen that requires further analysis.

Methods: Fourteen azole resistant Candida glabrata clinical isolates were collected from a Lebanese hospital and analysed through whole genome sequencing for single nucleotide polymorphisms in key resistance and virulence genes, and for phylogenetic relatedness. Isolates were then characterised for pathogenicity related attributes.

Results: All isolates had Lys314Glu mutation in ERG20 with multiple isolates displaying numerous shared mutations, such as Glu291Lys in CDR2 and Ala16Thr in CDR3. With the exception of two isolates that clustered together, most isolates were over 99.6% identical based on a genomic heatmap, implying high relatedness consistent with localised clonal expansion, although SNP differences appeared too high to support this. However, the isolates exhibited increased ergosterol and chitin content, as well as upregulation of drug efflux pumps resulting in drug resistance.

Conclusion: Our hospital isolates showed convergent resistant pathways, with many isolates having both shared and unique mutations and a high degree of genomic similarities.

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综合基因组和表型分析揭示临床热带假丝酵母分离株的趋同耐药模式。

背景：医院环境中热带念珠菌分离的发病率正在上升。对唑的高耐药性和死亡率使其成为一种需要进一步分析的病原体。方法：从黎巴嫩某医院收集14株耐唑光假丝酵母临床分离株，通过全基因组测序分析关键耐药和毒力基因的单核苷酸多态性及系统发育亲缘关系。然后对分离物的致病性相关属性进行表征。结果：所有分离株在ERG20中均存在Lys314Glu突变，多个分离株在CDR2中存在Glu291Lys突变，在CDR3中存在Ala16Thr突变。除了聚集在一起的两个分离株外，根据基因组热图，大多数分离株的相似性超过99.6%，这意味着与局部克隆扩增一致的高度亲缘性，尽管SNP差异似乎太高而无法支持这一点。然而，分离株显示麦角甾醇和几丁质含量增加，以及药物外排泵上调导致耐药性。结论：我们医院的分离株表现出趋同的耐药途径，许多分离株具有共同和独特的突变以及高度的基因组相似性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Mycoses 医学-皮肤病学

CiteScore

10.00

自引率

8.20%

发文量

143

审稿时长

6-12 weeks

期刊介绍： The journal Mycoses provides an international forum for original papers in English on the pathogenesis, diagnosis, therapy, prophylaxis, and epidemiology of fungal infectious diseases in humans as well as on the biology of pathogenic fungi. Medical mycology as part of medical microbiology is advancing rapidly. Effective therapeutic strategies are already available in chemotherapy and are being further developed. Their application requires reliable laboratory diagnostic techniques, which, in turn, result from mycological basic research. Opportunistic mycoses vary greatly in their clinical and pathological symptoms, because the underlying disease of a patient at risk decisively determines their symptomatology and progress. The journal Mycoses is therefore of interest to scientists in fundamental mycological research, mycological laboratory diagnosticians and clinicians interested in fungal infections.