From research to application: evaluation of literature-based and newly identified GWAS and GP-derived loci for anthracnose resistance in white lupin, across validation panels and environments.
András Patyi, Michael Schneider, Christine Arncken, Michał Książkiewicz, Monika M Messmer, Grit Schwertfirm, Mariateresa Lazzaro
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引用次数: 0
Abstract
Resistance to anthracnose disease, caused by Colletotrichum lupini, in white lupin is polygenic and rare in global germplasm, making breeding for resistance challenging. Several studies have identified genomic regions associated with resistance, but reported QTLs rarely overlap. Overall, anthracnose-associated loci have not yet been appropriately exploited in breeding due to the lack of validation, especially in unrelated material. We assessed three literature-based SNP-markers associated to a previously described QTL, as well as six originating from two published GWAS studies. In addition, by re-analysing two published datasets, we newly (i) identified five SNP-markers through a genome-wide association study (GWAS) and (ii) assembled a genomic prediction (GP) model based on a set of 42 SNP-markers. All SNP-markers were transformed to PCR-based assays to facilitate cost-efficient application in breeding and tested in a diversity panel and two breeding panels, phenotyped in field and/or controlled conditions. We validated the association to anthracnose resistance for the previously reported QTL on chromosome Lalb_Chr10, tagging a source of resistance in the resistant cultivar 'Frieda'. Two markers showed significant associations (p < 0.05) with anthracnose resistance in at least two panels, and an additional seven markers were significant in one. The GP model had a prediction accuracy of 0.84 (± 0.13) in the training panel, but is not applicable as such in the unrelated validation panels. However, re-training the GP model with the respective validation panel data showed clear prediction improvement buffering the limitation of environmental context and narrower genepools applied in a breeding programme.
Supplementary information: The online version contains supplementary material available at 10.1007/s11032-026-01661-w.
期刊介绍：
Molecular Breeding is an international journal publishing papers on applications of plant molecular biology, i.e., research most likely leading to practical applications. The practical applications might relate to the Developing as well as the industrialised World and have demonstrable benefits for the seed industry, farmers, processing industry, the environment and the consumer.
All papers published should contribute to the understanding and progress of modern plant breeding, encompassing the scientific disciplines of molecular biology, biochemistry, genetics, physiology, pathology, plant breeding, and ecology among others.
Molecular Breeding welcomes the following categories of papers: full papers, short communications, papers describing novel methods and review papers. All submission will be subject to peer review ensuring the highest possible scientific quality standards.
Molecular Breeding core areas:
Molecular Breeding will consider manuscripts describing contemporary methods of molecular genetics and genomic analysis, structural and functional genomics in crops, proteomics and metabolic profiling, abiotic stress and field evaluation of transgenic crops containing particular traits. Manuscripts on marker assisted breeding are also of major interest, in particular novel approaches and new results of marker assisted breeding, QTL cloning, integration of conventional and marker assisted breeding, and QTL studies in crop plants.
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