Antifungal resistance and Ocimum gratissimum L. essential oil-clotrimazole interactions in clinical Candida isolates from Vietnam.

IF 2.4 4区生物学 Q3 MICROBIOLOGY

Future microbiology Pub Date : 2026-04-01 Epub Date: 2026-04-28 DOI:10.1080/17460913.2026.2665051

Dang Anh Tuan, Jan Masak

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引用次数: 0

Abstract

Aims: To describe Candida species distribution and antifungal susceptibility in a Vietnamese tertiary-hospital collection and to evaluate Ocimum gratissimum L. essential oil (OGEO) as an in-vitro adjunct to clotrimazole.

Materials and methods: We retrospectively analysed 423 clinical Candida isolates collected in 2017-2018. Species identification used routine phenotypic methods/VITEK 2; susceptibility to fluconazole, voriconazole, and amphotericin B followed CLSI M27-A3. OGEO was chemically profiled by GC-MS. Crystal violet assays assessed OGEO inhibition of biofilm formation in 30 C. albicans isolates, and checkerboard assays evaluated OGEO-clotrimazole interactions in 20 species-resolved isolates using FICI.

Results: Species-level identification was available for 212 isolates; C. tropicalis (25.8% of the full cohort) slightly exceeded C. albicans (21.8%), while 49.8% were recorded as Candida spp. Overall fluconazole resistance was 13.7%, with higher resistance in C. tropicalis than C. albicans. OGEO was eugenol-rich (66.7%) and inhibited C. albicans biofilm biomass by 71.2% at 1% and 89.6% at 2% v/v. OGEO-clotrimazole interactions were FICI-defined synergistic in 65%, additive in 30%, indifferent in 5%, and never antagonistic.

Conclusion: OGEO showed promising in-vitro anti-biofilm and clotrimazole-potentiating activity, supporting further standardized, species-matched, topical-formulation and safety studies.

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越南临床假丝酵母菌分离株的抗真菌耐药性及其精油与克霉唑的相互作用。

目的：描述越南三甲医院收集的念珠菌种类分布和抗真菌敏感性，并评价芫花精油（Ocimum gratissimum L.精油）作为克霉唑的体外佐剂。材料与方法：回顾性分析2017-2018年收集的423株临床念珠菌分离株。物种鉴定采用常规表型方法/VITEK 2；CLSI M27-A3对氟康唑、伏立康唑和两性霉素B的敏感性。用GC-MS对OGEO进行化学分析。结晶紫法评估了30株白色念珠菌分离株中OGEO对生物膜形成的抑制作用，棋盘法评估了20株FICI分离株中OGEO-氯霉唑的相互作用。结果：212株分离物获得种级鉴定；热带假丝酵母菌（25.8%）略高于白色假丝酵母菌（21.8%），假丝酵母菌（49.8%）高于热带假丝酵母菌（25.8%），氟康唑整体耐药性为13.7%，热带假丝酵母菌高于白色假丝酵母菌。OGEO富含丁香酚（66.7%），对白色念珠菌生物膜生物量的抑制作用分别为1%和2% v/v时的71.2%和89.6%。ogeo -氯霉唑相互作用65%为fci定义的增效作用，30%为加性作用，5%为无差异作用，且无拮抗作用。结论：OGEO具有良好的体外抗生物膜和增强氯霉唑的活性，支持进一步的标准化、物种匹配、局部配方和安全性研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Future microbiology 生物-微生物学

CiteScore

4.90

自引率

3.20%

发文量

134

审稿时长

6-12 weeks

期刊介绍： Future Microbiology delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for this increasingly important and vast area of research.