{"title":"Structural Characterization and Anti-Inflammatory Activities of Polysaccharide From Hericium erinaceus Mycelium.","authors":"Chong Sun, Liuya Wang, Yushan Shi, Xueqing Geng, Huiqing Sun, Tengda Li, Qi Zhu, Lanfeng Hui, Xiaojian Wu, Lijing Chen, Zhenyuan Zhu","doi":"10.1002/bab.70148","DOIUrl":null,"url":null,"abstract":"<p><p>A novel heteropolysaccharide named HEP-S was separated from the mycelium of Hericium erinaceus using hot water extraction. The structure of HEP-S (2.45 × 10<sup>6</sup> Da) is mainly composed of →4,6)-α-D-Glcp(1→, →2)-α-D-Manp(1→, →4)-α-D-Glcp(1→, →3,6)-β-D-Glcp(1→, T-β-D-Galp(1→, and →4)-β-D-Galp(1→. In addition, we investigated the anti-inflammatory activity and mechanism of HEP-S in lipopolysaccharide-stimulated RAW264.7 cells. HEP-S (20, 40, and 80 µg/mL) could suppress the production of inflammatory cytokines by reducing vital transcription factors in the nuclear factor-κB signaling (NF-κB) pathway. The results showed that HEP-S exhibited notable anti-inflammatory activity on RAW 264.7, demonstrating its potential therapeutic effect on relieving gastritis.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":"1975-1987"},"PeriodicalIF":2.5000,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13446425/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biotechnology and applied biochemistry","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1002/bab.70148","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2026/4/24 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
A novel heteropolysaccharide named HEP-S was separated from the mycelium of Hericium erinaceus using hot water extraction. The structure of HEP-S (2.45 × 106 Da) is mainly composed of →4,6)-α-D-Glcp(1→, →2)-α-D-Manp(1→, →4)-α-D-Glcp(1→, →3,6)-β-D-Glcp(1→, T-β-D-Galp(1→, and →4)-β-D-Galp(1→. In addition, we investigated the anti-inflammatory activity and mechanism of HEP-S in lipopolysaccharide-stimulated RAW264.7 cells. HEP-S (20, 40, and 80 µg/mL) could suppress the production of inflammatory cytokines by reducing vital transcription factors in the nuclear factor-κB signaling (NF-κB) pathway. The results showed that HEP-S exhibited notable anti-inflammatory activity on RAW 264.7, demonstrating its potential therapeutic effect on relieving gastritis.
采用热水萃取法从猴头菌菌丝体中分离出一种新的杂多糖HEP-S。HEP-S的结构(2.45×106 Da)主要由→4、6)-α-D-Glcp(1→→2)-α-D-Manp(1→→4)-α-D-Glcp(1→→3、6)-β-D-Glcp T -β(1→-D-Galp(1→→4)-β-D-Galp(1→。此外，我们还研究了HEP-S在脂多糖刺激的RAW264.7细胞中的抗炎活性及其机制。HEP-S（20、40和80µg/mL）可通过降低核因子-κB信号通路中的重要转录因子来抑制炎症细胞因子的产生。结果显示，HEP-S对RAW 264.7具有显著的抗炎活性，表明其具有缓解胃炎的潜在治疗作用。
期刊介绍：
Published since 1979, Biotechnology and Applied Biochemistry is dedicated to the rapid publication of high quality, significant research at the interface between life sciences and their technological exploitation.
The Editors will consider papers for publication based on their novelty and impact as well as their contribution to the advancement of medical biotechnology and industrial biotechnology, covering cutting-edge research in synthetic biology, systems biology, metabolic engineering, bioengineering, biomaterials, biosensing, and nano-biotechnology.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
