Identifying Target Genes for Engineered Genetic Incompatibility in Fish

Abstract

Genetic biocontrol approaches promise to complement existing physical and chemical methods as part of integrated pest management (IPM) strategies for the control of aquatic invasive species (AIS). Engineered Genetic Incompatibility (EGI) is a strategy for producing organisms that are reproductively isolated from wild conspecifics and could be used as non-persistent genetic biocontrol agents. Previously successfully demonstrated in yeast and insects, here we report early-stage research and development results towards translating this approach from insects into fish. Using Danio rerio (zebrafish) as a model system, we report the identification of target genes and sequence-programmable transcriptional activators (PTAs) that are suitable for EGI development in fish and evaluate their performance in vivo, in a model organism. We also describe several challenges faced when integrating the component parts into a complete system capable of displaying complete genetic incompatibility with wild-type conspecifics. Lastly, we discuss the steps needed to translate EGI from a model fish species to a target invasive species such as common carp (Cyprinus carpio).