Serum miR-21 and miR-192 levels in type 2 diabetes: Association with albuminuria and renal function

4区医学 Q3 Nursing

Endocrinologia, Diabetes y Nutricion Pub Date : 2026-05-01 Epub Date: 2025-11-26 DOI:10.1016/j.endinu.2025.501721

Nosaiba M. Abd Alrahim , Alshaymaa A. Abd Elalim , Enas A. Farrag , Shimaa M. Abdel-Kareem , Sahar SaadEldeen Shaban , Aya A. Eldamanhory , Sammar Ahmed Kasim , Radwa M. Fath Allah , Fatma I. Mayah , Aya A. Ghamry , Sabah M. Alkhawagah , Emad Gamil Khidr

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引用次数: 0

Abstract

Background and aims

Albuminuria is a widely used clinical marker for diabetic nephropathy (DN); however, its limited sensitivity and specificity in detecting early renal alterations highlight the need for additional indicators. MicroRNAs (miRNAs) are emerging as molecular regulators involved in the pathogenesis of DN and may provide insight into early renal involvement in type 2 diabetes mellitus (T2DM). This study aimed to evaluate the serum expression levels of miR-21 and miR-192 in normo- and microalbuminuric patients with T2DM, and explore their associations with metabolic and renal parameters.

Methods

This case–control study included 104 individuals, including 60 patients with T2DM and 44 age- and sex-matched healthy controls. Individuals with diabetes were further classified into 30 normoalbuminuric and 30 microalbuminuric individuals based on the urinary albumin-to-creatinine ratio. Serum levels of miR-21 and miR-192 were quantified using real-time quantitative reverse transcription PCR.

Results

Both miR-21 and miR-192 were significantly upregulated in Patients with T2DM vs healthy controls. Notably, miR-21 expression exhibited a stepwise increase from normo- to microalbuminuria and showed strong correlations with metabolic and renal parameters, including HbA1c, ACR, and lipid profile. In ROC analysis, miR-21 demonstrated better discriminative ability between study groups vs miR-192.

Conclusion

Serum miR-21 may reflect early renal involvement in patients with T2DM. Its progressive increase with albuminuria and strong associations with clinical parameters suggest potential value as a supportive molecular indicator in the early evaluation of diabetic nephropathy. Further prospective studies are needed to validate its clinical applicability.

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2型糖尿病患者血清miR-21和miR-192水平：与蛋白尿和肾功能的关系

背景与目的尿蛋白尿是糖尿病肾病（DN）的临床标志物；然而，它在检测早期肾脏改变方面的敏感性和特异性有限，因此需要更多的指标。MicroRNAs （miRNAs）正在成为参与DN发病机制的分子调控因子，并可能为2型糖尿病（T2DM）早期肾脏病变提供见解。本研究旨在评估正常和微量蛋白尿T2DM患者血清中miR-21和miR-192的表达水平，并探讨其与代谢和肾脏参数的关系。方法本病例对照研究纳入104例，其中60例为2型糖尿病患者，44例为年龄和性别匹配的健康对照。根据尿白蛋白与肌酐比值，将糖尿病患者进一步分为30例正常蛋白尿和30例微量蛋白尿。实时定量反转录PCR检测血清miR-21和miR-192水平。结果miR-21和miR-192在T2DM患者中与健康对照组相比均显著上调。值得注意的是，miR-21的表达从正常到微量白蛋白尿逐步增加，并与代谢和肾脏参数（包括HbA1c、ACR和脂质谱）有很强的相关性。在ROC分析中，与miR-192相比，miR-21在研究组之间表现出更好的区分能力。结论血清miR-21可能反映T2DM患者早期肾脏受累。它随着蛋白尿而逐渐增加，并与临床参数密切相关，这表明它作为早期评估糖尿病肾病的支持性分子指标具有潜在价值。需要进一步的前瞻性研究来验证其临床适用性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Endocrinologia, Diabetes y Nutricion Nursing-Nutrition and Dietetics

CiteScore

2.10

自引率

0.00%

发文量

169

审稿时长

35 days

期刊介绍： Endocrinología, Diabetes y Nutrición is the official journal of the Spanish Society of Endocrinology and Nutrition (Sociedad Española de Endocrinología y Nutrición, SEEN) and the Spanish Society of Diabetes (Sociedad Española de Diabetes, SED), and was founded in 1954. The aim of the journal is to improve knowledge and be a useful tool in practice for clinical and laboratory specialists, trainee physicians, researchers, and nurses interested in endocrinology, diabetes, nutrition and related disciplines. It is an international journal published in Spanish (print and online) and English (online), covering different fields of endocrinology and metabolism, including diabetes, obesity, and nutrition disorders, as well as the most relevant research produced mainly in Spanish language territories. The quality of the contents is ensured by a prestigious national and international board, and by a selected panel of specialists involved in a rigorous peer review. The result is that only manuscripts containing high quality research and with utmost interest for clinicians and professionals related in the field are published. The Journal publishes Original clinical and research articles, Reviews, Special articles, Clinical Guidelines, Position Statements from both societies and Letters to the editor. Endocrinología, Diabetes y Nutrición can be found at Science Citation Index Expanded, Medline/PubMed and SCOPUS.