Longitudinal Follow-Up of Gut and Salivary Microbiota in Children With Food Protein-Induced Enterocolitis Syndrome

IF 11.3 1区医学 Q1 ALLERGY

Allergy Pub Date : 2026-10-08 Epub Date: 2026-04-28 DOI:10.1111/all.70363

Anais Lemoine, Karine Adel-Patient, Nathalie Kapel, Camille Mayeur, Marie-Noelle Rossignol, Aurelia Bruneau, Patrick Tounian, Muriel Thomas, Ioannis Nicolis

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引用次数: 0

Abstract

Background

Gut microbiota and their metabolites play a key role in digestive dysimmunity and tolerance breakdown, leading to pathologies such as food protein-induced enterocolitis syndrome (FPIES). This study aimed to compare both the gut and salivary microbiota over time between the allergic and tolerant patients and the matched controls.

Methods

Faecal and salivary samples were collected longitudinally from FPIES allergic patients on an elimination diet, and after their natural tolerance acquisition, and from matched controls (1:1 ratio). 16S rRNA gene sequencing and biostatistical analyses (ALDEx2, ANCOM-BC, DESeq2, LEfSe, and MaAsLIN2) were used to compare microbiota composition between groups. The measurement of faecal short-chain fatty acids (SCFAs) using gas chromatography evaluated the gut microbiota functions.

Results

Thirty-eight allergic patients were included (median age: 1.3 years), and 22 became tolerant over time. Allergic patients exhibited lower gut and salivary alpha-diversities, and several different genera (12 in stools, including less Ruminococcus, and 2 in saliva), and higher relative abundance of acetate than the controls. With tolerance acquisition, faecal alpha-diversities partially increased, whereas SCFAs normalised. Ultimately, there were more differences in faecal and salivary microbiota in allergic patients versus after tolerance acquisition (i.e., more Blautia, Ruminococcus, Faecalibacterium, Prevotella-9, and less Escherichia–Shigella in tolerant patients) than between the controls over time.

Conclusion

The faecal and salivary dysbiosis observed in allergic patients with FPIES partially resolved after tolerance acquisition. Understanding the underlying mechanisms of dysbiosis is crucial to prevent and help manage FPIES. A prolonged follow-up could determine whether dysbiosis resolution may be complete, and whether this early infancy dysbiosis has long-term consequences on health.

查看原文本刊更多论文

食物蛋白诱导的小肠结肠炎综合征患儿肠道和唾液微生物群的纵向随访。

肠道菌群及其代谢物在消化免疫障碍和耐受性破坏中发挥关键作用，导致诸如食物蛋白诱导的小肠结肠炎综合征（FPIES）等病理。本研究旨在比较过敏和耐受患者与匹配对照组之间的肠道和唾液微生物群随时间的变化。方法纵向采集消除饮食、自然耐受性获得后和匹配对照（1:1比例）的FPIES过敏患者的粪便和唾液样本。采用16S rRNA基因测序和生物统计学分析（ALDEx2、ANCOM-BC、DESeq2、LEfSe和MaAsLIN2）比较各组微生物群组成。用气相色谱法测定粪便短链脂肪酸（SCFAs）评估肠道微生物群功能。结果纳入38例过敏患者（中位年龄：1.3岁），其中22例随着时间的推移变得耐受。过敏患者表现出较低的肠道和唾液α多样性，以及几个不同的属（粪便中有12个，包括较少的鲁米诺球菌，唾液中有2个），醋酸盐的相对丰度高于对照组。随着耐受性的获得，粪便α多样性部分增加，而scfa正常化。最终，随着时间的推移，过敏患者的粪便和唾液微生物群与获得耐受性后的差异更大（即，耐受性患者的Blautia， Ruminococcus, Faecalibacterium， Prevotella-9更多，而埃希氏杆菌-志贺氏菌更少）。结论在获得耐受性后，过敏患者的粪便和唾液生态失调得到部分缓解。了解生态失调的潜在机制对于预防和帮助管理FPIES至关重要。长期随访可以确定生态失调是否可以完全解决，以及这种早期婴儿生态失调是否对健康有长期影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Allergy 医学-过敏

CiteScore

26.10

自引率

9.70%

发文量

393

审稿时长

2 months

期刊介绍： Allergy is an international and multidisciplinary journal that aims to advance, impact, and communicate all aspects of the discipline of Allergy/Immunology. It publishes original articles, reviews, position papers, guidelines, editorials, news and commentaries, letters to the editors, and correspondences. The journal accepts articles based on their scientific merit and quality. Allergy seeks to maintain contact between basic and clinical Allergy/Immunology and encourages contributions from contributors and readers from all countries. In addition to its publication, Allergy also provides abstracting and indexing information. Some of the databases that include Allergy abstracts are Abstracts on Hygiene & Communicable Disease, Academic Search Alumni Edition, AgBiotech News & Information, AGRICOLA Database, Biological Abstracts, PubMed Dietary Supplement Subset, and Global Health, among others.