Potential pathogenesis of Helicobacter pylori strains from Cameroon: seroprevalence of 10 proteins involved in the pathogenesis of Helicobacter pylori-related gastro-duodenal diseases, measured using a new multiplex immunoassay among Helicobacter pylori IgM-positive dyspeptic patients.

IF 2.1 Q3 MEDICINE, RESEARCH & EXPERIMENTAL

Future Science OA Pub Date : 2026-12-01 Epub Date: 2026-04-18 DOI:10.1080/20565623.2026.2657525

Viny Adrien Fouomene Kenne, Laure Brigitte Kouitcheu Mabeku

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Abstract

Background: This study aims to evaluate the seroprevalence of Helicobacter pylori (H. pylori) proteins involved in its pathogenicity among H. pylori infected patients.

Research design and methods: The analyses were based on 272 serums from H. pylori IgM-positive dyspeptic patients. H. pylori GroEL, FliD, HpaA, gGT, CagA, VacA, NapA, CtkA, HtrA, and HP0231 proteins was detected using multiplex serology method. The detected H. pylori antigens were classified as antigens for colonization, chaperone systems, immunomodulatory factors and toxins/effectors delivery/T4SS-related support factors.

Results: H. pylori CagA, VacA, FliD, HpaA, GroEL gGT, HtrA, HP0231, CtkA, and NapA proteins were present in 97.1, 77.2, 77.2, 73.2, 61, 34.2, 25.4, 18.8, 11.4, and 9.9%. Antigens involved in colonization, immune response, proteins chaperone, and toxins/effectors and T4SS-related support factors were detected in 81.9%, 19.8%, 70.5%, and 10.6%, respectively. Significant high sensitivity of virulence antigens was recorded among patients aged more than 20 years old, those from west region, male, blood group O, and overweight/obese patients.

Conclusion: Our results showed that H. pylori strains in our milieu have a strong ability to colonize the gastric mucosa, but a low carcinogenic potential due to the scarcity of the proteins modulating the detrimental effect of CagA/VacA proteins.

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喀麦隆幽门螺杆菌菌株的潜在发病机制：在幽门螺杆菌igm阳性消化不良患者中，使用一种新的多重免疫分析法测量了与幽门螺杆菌相关的胃十二指肠疾病发病机制相关的10种蛋白质的血清阳性率。

背景：本研究旨在评估幽门螺杆菌感染患者中参与其致病性的幽门螺杆菌蛋白的血清阳性率。研究设计和方法：分析了272例幽门螺杆菌igm阳性消化不良患者的血清。采用多重血清学方法检测幽门螺杆菌GroEL、FliD、HpaA、gGT、CagA、VacA、NapA、CtkA、HtrA、HP0231蛋白。检测到的幽门螺杆菌抗原分为定植抗原、伴侣系统抗原、免疫调节因子抗原和毒素/效应器递送/ t4ss相关支持因子抗原。结果：幽门螺杆菌CagA、VacA、FliD、HpaA、GroEL gGT、HtrA、HP0231、CtkA、NapA蛋白含量分别为97.1、77.2、77.2、73.2、61、34.2、25.4、18.8、11.4、9.9%。定植抗原、免疫应答抗原、蛋白伴侣抗原、毒素/效应器抗原和t4ss相关支持因子的检出率分别为81.9%、19.8%、70.5%和10.6%。20岁以上、西部地区、男性、O型血、超重/肥胖患者对毒力抗原的敏感性较高。结论：我们的研究结果表明，我们环境中的幽门螺杆菌菌株具有很强的胃粘膜定植能力，但由于缺乏调节CagA/VacA蛋白有害作用的蛋白质，因此其致癌潜力较低。

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来源期刊

Future Science OA MEDICINE, RESEARCH & EXPERIMENTAL-

CiteScore

5.00

自引率

4.00%

发文量

审稿时长

13 weeks

期刊介绍： Future Science OA is an online, open access, peer-reviewed title from the Future Science Group. The journal covers research and discussion related to advances in biotechnology, medicine and health. The journal embraces the importance of publishing all good-quality research with the potential to further the progress of research in these fields. All original research articles will be considered that are within the journal''s scope, and have been conducted with scientific rigour and research integrity. The journal also features review articles, editorials and perspectives, providing readers with a leading source of commentary and analysis. Submissions of the following article types will be considered: -Research articles -Preliminary communications -Short communications -Methodologies -Trial design articles -Trial results (including early-phase and negative studies) -Reviews -Perspectives -Commentaries