A novel cytochrome P450 gene, CYP12A2, regulates reproduction of Mythimna separata by modulating 20E biosynthesis.

IF 3.8 1区农林科学 Q1 AGRONOMY

Pest Management Science Pub Date : 2026-04-18 DOI:10.1002/ps.70853

Lingling Li,Wenmeng Li,Jing Liao,Peiying Li,Changgeng Dai,Hongbo Li

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引用次数: 0

Abstract

BACKGROUND Several P450 genes in the 20-hydroxyecdysone (20E) signaling pathway are known to critically regulate reproductive development in insects. However, the contribution of other P450 family members to insect reproduction remains poorly characterized. RESULTS Here, we identified a novel P450 gene, CYP12A2, from the highly polyphagous pest Mythimna separata. Quantitative real-time polymerase chain reaction analysis revealed that CYP12A2 was upregulated during sexual maturation and predominantly enriched in the ovaries of 3-day-old female adults. RNA interference (RNAi)-mediated knockdown of CYP12A2 severely disrupted ovarian development and downregulated the expression of vitellogenesis-related genes (Vg and VgR) as well as eggshell formation-related genes (CB-Ld329 and FCP3C), ultimately leading to reduced fecundity. Moreover, silencing of CYP12A2 significantly decreased 20E titers and suppressed the expression of key 20E biosynthetic genes (CYP307A1, CYP306A1, CYP302A1 and CYP315A1). Importantly, exogenous 20E injection substantially rescued the defects in ovarian development and restored the expression of reproduction-related genes that were impaired by CYP12A2 depletion. CONCLUSION These findings indicate that CYP12A2 regulates reproduction of M. separata by modulating 20E biosynthesis. Our study uncovers a novel reproductive role for CYP12A2 and highlights its potential as a target for RNAi-based pest management strategies. © 2026 Society of Chemical Industry.

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一种新的细胞色素P450基因CYP12A2通过调节20E生物合成来调节Mythimna separata的繁殖。

20-羟基蜕皮激素（20E）信号通路中的几个P450基因已知对昆虫的生殖发育起关键调控作用。然而，其他P450家族成员对昆虫繁殖的贡献仍然知之甚少。结果从高食性害虫Mythimna separata中鉴定出一个新的P450基因CYP12A2。实时定量聚合酶链反应分析显示，CYP12A2在性成熟过程中表达上调，主要富集于3日龄雌性成虫的卵巢。RNA干扰（RNAi）介导的CYP12A2敲低严重破坏卵巢发育，下调卵黄形成相关基因（Vg、VgR）和蛋壳形成相关基因（CB-Ld329、FCP3C）的表达，最终导致繁殖力降低。此外，CYP12A2的沉默显著降低了20E滴度，抑制了20E关键生物合成基因（CYP307A1、CYP306A1、CYP302A1和CYP315A1）的表达。重要的是，外源性20E注射大大挽救了卵巢发育缺陷，恢复了因CYP12A2缺失而受损的生殖相关基因的表达。结论CYP12A2通过调节20E的生物合成来调节分离假体的繁殖。我们的研究揭示了CYP12A2的一种新的生殖作用，并强调了它作为基于rnai的害虫管理策略的目标的潜力。©2026化学工业协会。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Pest Management Science 农林科学-昆虫学

CiteScore

7.90

自引率

9.80%

发文量

553

审稿时长

4.8 months

期刊介绍： Pest Management Science is the international journal of research and development in crop protection and pest control. Since its launch in 1970, the journal has become the premier forum for papers on the discovery, application, and impact on the environment of products and strategies designed for pest management. Published for SCI by John Wiley & Sons Ltd.