Genomic Characterization of a Locally Transmitted Leishmania mexicana Isolate from Texas.

IF 1.6 4区医学 Q3 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH

American Journal of Tropical Medicine and Hygiene Pub Date : 2026-04-16 DOI:10.4269/ajtmh.25-0744

Juan David Ramírez, Luz H Patiño, Binita Nepal, Sarah M Gunter, Eva H Clark, Dawn M Wetzel

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Abstract

Cutaneous leishmaniasis, which is caused by Leishmania species transmitted by sandflies, is increasingly recognized in subtropical, higher-resource regions, including the southern United States. The first whole-genome sequence of Leishmania mexicana (L. mexicana) is reported from an autochthonous US case in a 3-year-old boy from Ellis County, Texas, with no travel history. Genomic DNA was sequenced using Illumina (San Diego, CA) technology. Phylogenomic analysis of nuclear and mitochondrial single-nucleotide polymorphisms (SNPs) confirmed the isolate as L. mexicana, clustering within the L. mexicana complex. The parasite displayed a predominantly disomic karyotype, with chromosome 30 exhibiting trisomy. A total of 172 genes were identified, with notable copy number variation and 9,920 SNPs unique to this genome, highlighting substantial genetic diversity. These variants affected genes involved in host-pathogen interactions, metabolism, and signaling. Overall, this study emphasizes the need for molecular surveillance in regions where competent sandfly vectors are present.

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德克萨斯州本地传播的墨西哥利什曼原虫分离株的基因组特征。

皮肤利什曼病是由白蛉传播的利什曼原虫引起的，在包括美国南部在内的亚热带资源较高地区日益得到认可。墨西哥利什曼原虫（L. mexicana）的首个全基因组序列报告来自美国德克萨斯州埃利斯县一名无旅行史的3岁男孩的本土病例。基因组DNA测序采用Illumina （San Diego， CA）技术。核和线粒体单核苷酸多态性（SNPs）系统基因组分析证实该分离物为墨西哥乳杆菌，聚类在墨西哥乳杆菌复合体内。寄生虫主要表现为二染色体核型，第30号染色体表现为三染色体。共鉴定出172个基因，拷贝数差异显著，该基因组特有的SNPs达9920个，显示了丰富的遗传多样性。这些变异影响了参与宿主-病原体相互作用、代谢和信号传导的基因。总的来说，这项研究强调了在有白蛉病媒存在的地区进行分子监测的必要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

American Journal of Tropical Medicine and Hygiene 医学-公共卫生、环境卫生与职业卫生

CiteScore

6.20

自引率

3.00%

发文量

508

审稿时长

3 months

期刊介绍： The American Journal of Tropical Medicine and Hygiene, established in 1921, is published monthly by the American Society of Tropical Medicine and Hygiene. It is among the top-ranked tropical medicine journals in the world publishing original scientific articles and the latest science covering new research with an emphasis on population, clinical and laboratory science and the application of technology in the fields of tropical medicine, parasitology, immunology, infectious diseases, epidemiology, basic and molecular biology, virology and international medicine. The Journal publishes unsolicited peer-reviewed manuscripts, review articles, short reports, images in Clinical Tropical Medicine, case studies, reports on the efficacy of new drugs and methods of treatment, prevention and control methodologies,new testing methods and equipment, book reports and Letters to the Editor. Topics range from applied epidemiology in such relevant areas as AIDS to the molecular biology of vaccine development. The Journal is of interest to epidemiologists, parasitologists, virologists, clinicians, entomologists and public health officials who are concerned with health issues of the tropics, developing nations and emerging infectious diseases. Major granting institutions including philanthropic and governmental institutions active in the public health field, and medical and scientific libraries throughout the world purchase the Journal. Two or more supplements to the Journal on topics of special interest are published annually. These supplements represent comprehensive and multidisciplinary discussions of issues of concern to tropical disease specialists and health issues of developing countries