Tumor-targeting bioluminescent bacteria for in vivo imaging.

Engineering Microbiology Pub Date : 2025-07-11 eCollection Date: 2025-09-01 DOI:10.1016/j.engmic.2025.100224

Chenghao Ma, Jingxi Liu, Hongjing Liu, Xiaohan Zhao, Geng Li, Youming Zhang, Tianyu Jiang

{"title":"Tumor-targeting bioluminescent bacteria for in vivo imaging.","authors":"Chenghao Ma, Jingxi Liu, Hongjing Liu, Xiaohan Zhao, Geng Li, Youming Zhang, Tianyu Jiang","doi":"10.1016/j.engmic.2025.100224","DOIUrl":null,"url":null,"abstract":"As the understanding of bacteria-mediated cancer therapies deepens, bacteria such as Escherichia coli Nissle 1917 (EcN) have become a promising platform for cancer therapy. However, their potential role in real-time monitoring and visualization tools still needs to be explored and enhanced. In this study, we aimed to screen and optimize EcN visualization systems for non-invasive in vivo bioluminescence imaging in live mice. To this end, we developed three series of recombinant EcN strains expressing Gaussia luciferase (Gluc), Renilla luciferase (Rluc), and NanoLuc (Nluc), along with their respective mutants. These strains exhibited bioluminescence when different coelenterazine (CTZ) substrates were present. As a result, multiple bioluminescent EcN strain-substrate pairs were identified with stronger, longer, or red-shifted bioluminescence, offering multiple effective optical tumor-targeting systems for in vivo studies investigating bacteria-mediated cancer therapy and intestinal diseases.","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"5 3","pages":"100224"},"PeriodicalIF":0.0000,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12967832/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Engineering Microbiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1016/j.engmic.2025.100224","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/9/1 0:00:00","PubModel":"eCollection","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

Abstract

As the understanding of bacteria-mediated cancer therapies deepens, bacteria such as Escherichia coli Nissle 1917 (EcN) have become a promising platform for cancer therapy. However, their potential role in real-time monitoring and visualization tools still needs to be explored and enhanced. In this study, we aimed to screen and optimize EcN visualization systems for non-invasive in vivo bioluminescence imaging in live mice. To this end, we developed three series of recombinant EcN strains expressing Gaussia luciferase (Gluc), Renilla luciferase (Rluc), and NanoLuc (Nluc), along with their respective mutants. These strains exhibited bioluminescence when different coelenterazine (CTZ) substrates were present. As a result, multiple bioluminescent EcN strain-substrate pairs were identified with stronger, longer, or red-shifted bioluminescence, offering multiple effective optical tumor-targeting systems for in vivo studies investigating bacteria-mediated cancer therapy and intestinal diseases.

查看原文本刊更多论文

肿瘤靶向生物发光细菌在体内成像。

随着对细菌介导的癌症治疗认识的加深，大肠杆菌Nissle 1917 （EcN）等细菌已成为癌症治疗的一个有前景的平台。然而，它们在实时监控和可视化工具中的潜在作用仍有待探索和增强。在这项研究中，我们旨在筛选和优化用于活体小鼠非侵入性体内生物发光成像的EcN可视化系统。为此，我们开发了三个系列的重组EcN菌株，分别表达Gaussia荧光素酶（Gluc）、Renilla荧光素酶（Rluc）和NanoLuc （Nluc）及其各自的突变体。当不同的coelenterazine （CTZ）底物存在时，这些菌株表现出生物发光。因此，多种生物发光EcN菌株-底物对被鉴定出具有更强、更长或红移的生物发光，为研究细菌介导的癌症治疗和肠道疾病的体内研究提供了多种有效的光学肿瘤靶向系统。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Engineering Microbiology

CiteScore

3.90

自引率

0.00%

发文量