Derepression of a Subset of Meiotic Proteins in Primordial Germ Cells of max Mutant Zebrafish.

IF 1.5 4区生物学 Q4 CELL BIOLOGY

Development Growth & Differentiation Pub Date : 2026-04-01 DOI:10.1111/dgd.70053

Yuya Hasegawa, Toshihiro Kawasaki, Yukiko Imai, Yasuhisa Matsui, Noriyoshi Sakai

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引用次数: 0

Abstract

Germ cells in zebrafish are specified by a preformation mechanism, unlike in mice. Max (Myc associated factor X) and its associated PRC1.6 (polycomb repressive complex 1.6) act as negative regulators of germ cell- and meiosis-related genes in mice; however, their function in zebrafish remains unknown. Here, we generated max mutants in zebrafish using genome-editing techniques to investigate the role of Max in germ cell development and meiosis. No obvious morphological abnormalities were observed in the mutants up to 4 days post fertilization (dpf), likely due to maternally supplied max mRNAs. However, the mutants failed to form an inflated swim bladder and gradually died after 10 dpf. The number of primordial germ cells (PGCs) did not differ between the max mutant and wild-type larvae up to 12 dpf. In contrast, analysis of protein expression levels of germ cell-related and meiosis-related genes in PGCs at 9 dpf revealed that the levels of Sycp2, Sycp3, and Dmc1 proteins were significantly increased in max mutants, whereas the expression of Ddx4 and Piwil1 remained unchanged. Together, these results suggest that zebrafish Max selectively represses the expression of a subset of meiotic genes in PGCs.

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最大突变斑马鱼原始生殖细胞中减数分裂蛋白亚群的表达。

与小鼠不同，斑马鱼的生殖细胞是由一种预先形成机制指定的。Max （Myc相关因子X）及其相关的PRC1.6（多梳抑制复合体1.6）在小鼠生殖细胞和减数分裂相关基因中起负调控作用；然而，它们在斑马鱼中的功能尚不清楚。在这里，我们使用基因组编辑技术在斑马鱼中产生max突变体，以研究max在生殖细胞发育和减数分裂中的作用。在受精后4天（dpf），突变体未观察到明显的形态异常，可能是由于母体提供的最大mrna。然而，突变体未能形成膨胀的鱼鳔，并在10 dpf后逐渐死亡。原始生殖细胞（PGCs）的数量在最大突变体和野生型幼虫之间没有差异，可达12 dpf。相比之下，9 dpf时PGCs中生殖细胞相关基因和减数分裂相关基因的蛋白表达水平分析显示，在max突变体中，Sycp2、Sycp3和Dmc1蛋白的表达水平显著增加，而Ddx4和Piwil1的表达保持不变。总之，这些结果表明斑马鱼Max选择性地抑制PGCs中减数分裂基因子集的表达。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Development Growth & Differentiation 生物-发育生物学

CiteScore

4.60

自引率

4.00%

发文量

审稿时长

6 months

期刊介绍： Development Growth & Differentiation (DGD) publishes three types of articles: original, resource, and review papers. Original papers are on any subjects having a context in development, growth, and differentiation processes in animals, plants, and microorganisms, dealing with molecular, genetic, cellular and organismal phenomena including metamorphosis and regeneration, while using experimental, theoretical, and bioinformatic approaches. Papers on other related fields are also welcome, such as stem cell biology, genomics, neuroscience, Evodevo, Ecodevo, and medical science as well as related methodology (new or revised techniques) and bioresources. Resource papers describe a dataset, such as whole genome sequences and expressed sequence tags (ESTs), with some biological insights, which should be valuable for studying the subjects as mentioned above. Submission of review papers is also encouraged, especially those providing a new scope based on the authors’ own study, or a summarization of their study series.