Histological effects of intranasal clove essential oil on the olfactory bulb in a rat model of depression.

IF 3.1 4区生物学 Q1 ANATOMY & MORPHOLOGY

Tissue & cell Pub Date : 2026-08-01 Epub Date: 2026-04-08 DOI:10.1016/j.tice.2026.103508

Walaa Elfakharany, Majorelle Magdy Shafik, Mariam Asaad Amin, Marian Mokhtar

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引用次数: 0

Abstract

Background: depression is the most widespread psychiatric disorder, making it the fourth leading cause of death among adults. The olfactory system is a component of the limbic system. Repeated exposure to stressful events has been reported to affect emotions and olfactory perception. Clove oil is an essential oil that exhibits immunomodulatory properties and is used in the recovery of olfactory dysfunctions during the COVID-19 outbreak.

Aim: Detect the potential hazards of stress-induced depression on the olfactory bulbs and investigate the possible role of clove oil in ameliorating these effects.

Material and methods: Twenty-four adult male albino rats were divided equally into three groups: a control group (I), Group (II): depression group, rats were subjected to CUMS Model for 21 days, then were subdivided equally into group (IIa): rats were sacrificed after 21 days, and group (IIb): rats were left for spontaneous recovery for 14 days, then sacrificed. Group (III): rats were subjected to CUMS Model for 21 days, then clove was applied intranasally (0.02 mL/kg) daily for 14 days, then sacrificed. Olfactory bulb specimens were examined by light microscopy. The depression altered the normal tissue morphology of the bulb; Glomeruli appeared shrunken in the glomerular layer. Mitral cells appeared fewer in number, with deeply stained nuclei. The granule cells were aggregated with areas of vacuolated neuropil. Clove oil group restored the normal architecture of the olfactory bulb.

Conclusion: Clove oil has an ameliorating effect on the injury of the olfactory bulb in the depression induced animal model of Albino rats.

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丁香精油对大鼠抑郁模型嗅球的组织学影响。

背景：抑郁症是最普遍的精神疾病，使其成为成年人死亡的第四大原因。嗅觉系统是边缘系统的一个组成部分。据报道，反复接触压力事件会影响情绪和嗅觉。丁香油是一种具有免疫调节特性的精油，在COVID-19疫情期间用于恢复嗅觉功能障碍。目的：探讨应激性抑郁对嗅球的潜在危害，探讨丁香油在缓解应激性抑郁中的作用。材料与方法：将24只成年雄性白化大鼠平均分为3组：对照组(I)、抑郁组（II），建立CUMS模型21 d后再平均分为IIa组：21 d后处死，IIb组：自行恢复14 d后处死。第三组：造模21 d后，丁香灌胃（0.02 mL/kg），灌胃14 d后处死。用光学显微镜检查嗅球标本。凹陷改变了鳞茎的正常组织形态；肾小球层出现肾小球萎缩。二尖瓣细胞数量较少，细胞核染色深。颗粒细胞聚集在空泡化的神经细胞区。丁香油组恢复了嗅球的正常结构。结论：丁香油对白化病大鼠抑郁动物模型嗅球损伤有改善作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Tissue & cell 医学-解剖学与形态学

CiteScore

3.90

自引率

0.00%

发文量

234

期刊介绍： Tissue and Cell is devoted to original research on the organization of cells, subcellular and extracellular components at all levels, including the grouping and interrelations of cells in tissues and organs. The journal encourages submission of ultrastructural studies that provide novel insights into structure, function and physiology of cells and tissues, in health and disease. Bioengineering and stem cells studies focused on the description of morphological and/or histological data are also welcomed. Studies investigating the effect of compounds and/or substances on structure of cells and tissues are generally outside the scope of this journal. For consideration, studies should contain a clear rationale on the use of (a) given substance(s), have a compelling morphological and structural focus and present novel incremental findings from previous literature.