Evaluating SEPHS2 expression and glutathione peroxidase as biomarkers in recurrent pregnancy loss: a new insight.

IF 2.2 4区医学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biomarkers Pub Date : 2026-06-01 Epub Date: 2026-04-22 DOI:10.1080/1354750X.2026.2659525

Mai M Shaker, Eman Reda Galal, Taghreed A Shalabi, Nesma M Elaraby, Fatma Elzahraa Sobhy Ammar, Fatma Alzahraa Mohamed Hassan

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引用次数: 0

Abstract

Background: Selenophosphate synthetase 2 (SEPHS2) activates selenophosphate, a donor for selenocysteine required for glutathione peroxidase (GPX) production. GPX protects placental tissue. Dysregulation of SEPHS2 and reduced GPX activity may contribute to oxidative stress-mediated placental dysfunction in recurrent pregnancy loss (RPL).

Aim: To evaluate SEPHS2 gene expression and plasma GPX levels as prognostic biomarkers in women with idiopathic RPL and their association with oxidative imbalance.

Methods: A total of 100 women with idiopathic RPL and 100 healthy women without history of RPL were included. SEPHS2 gene expression was quantified using quantitative reverse transcription polymerase chain reaction (qRT-PCR), and plasma GPX levels were measured using enzyme-linked immunosorbent assay (ELISA).

Results: SEPHS2 expression was significantly elevated in the RPL group compared with controls (mean fold change: 14 ± 11.4 vs. 5 ± 8), while plasma GPx levels were markedly reduced in women with RPL (5.57 ± 0.40 vs. 9.53 ± 3.28; P < 0.001, 95% CI: 3.32-4.62). These findings indicate an association between increased SEPHS2 expression, reduced antioxidant activity, and recurrent pregnancy loss.

Conclusion: Elevated SEPHS2 expression suggests disrupted selenoprotein-mediated antioxidant defense in RPL. SEPHS2 and GPX may serve as prognostic biomarkers.

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评估SEPHS2表达和谷胱甘肽过氧化物酶作为复发性妊娠丢失的生物标志物：新的见解。

背景：硒磷酸合成酶2 （SEPHS2）激活硒磷酸，硒磷酸是谷胱甘肽过氧化物酶（GPX）生产所需的硒半胱氨酸的供体。GPX保护胎盘组织。在复发性妊娠丢失（RPL）中，SEPHS2的失调和GPX活性的降低可能导致氧化应激介导的胎盘功能障碍。目的：探讨SEPHS2基因表达和血浆GPX水平作为特发性RPL患者预后的生物标志物及其与氧化失衡的关系。方法：共纳入100例特发性RPL女性和100例无RPL病史的健康女性。采用定量逆转录聚合酶链反应（qRT-PCR）定量检测SEPHS2基因表达，酶联免疫吸附法（ELISA）检测血浆GPX水平。结果：与对照组相比，RPL组SEPHS2表达显著升高（平均倍数变化：14±11.4比5±8），而RPL组血浆GPx水平显著降低（5.57±0.40比9.53±3.28;P < 0.001, 95% CI: 3.32-4.62）。这些发现表明SEPHS2表达增加、抗氧化活性降低和复发性妊娠丢失之间存在关联。结论：SEPHS2表达升高提示硒蛋白介导的RPL抗氧化防御被破坏。SEPHS2和GPX可作为预后生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biomarkers 医学-毒理学

CiteScore

5.00

自引率

3.80%

发文量

140

审稿时长

3 months

期刊介绍： The journal Biomarkers brings together all aspects of the rapidly growing field of biomarker research, encompassing their various uses and applications in one essential source. Biomarkers provides a vital forum for the exchange of ideas and concepts in all areas of biomarker research. High quality papers in four main areas are accepted and manuscripts describing novel biomarkers and their subsequent validation are especially encouraged: • Biomarkers of disease • Biomarkers of exposure • Biomarkers of response • Biomarkers of susceptibility Manuscripts can describe biomarkers measured in humans or other animals in vivo or in vitro. Biomarkers will consider publishing negative data from studies of biomarkers of susceptibility in human populations.