Ammi majus as a New Natural Host of Bean Common Mosaic Virus

IF 1.2 4区农林科学 Q3 PLANT SCIENCES

Journal of Phytopathology Pub Date : 2026-03-29 DOI:10.1111/jph.70294

Vinicius Henrique Bello, Harri José Lorenzi, Jorge Alberto Marques Rezende, Elliot Watanabe Kitajima

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Abstract

In 2023, two Ammi majus plants showing mosaic symptoms were observed in the state of São Paulo, Brazil. Transmission electron microscope analysis of leaf extracts revealed the consistent presence of elongated, flexuous filamentous particles measuring 13–15 nm in width and 700–750 nm in length. Furthermore, analysis of ultrathin sections showed cytoplasmic cylindrical inclusions, characteristic of viruses of the genus Potyvirus. Molecular assays using universal primers, BCMV-specific primers, and amplicon sequencing confirmed the presence of bean common mosaic virus (BCMV, Potyvirus phaseovulgaris). Phylogenetic analysis grouped the BCMV isolate from A. majus (BCMV-Am) in a clade with BCMV isolates from Aristolochia spp. plants. Mechanical inoculation assays resulted in chlorotic local lesions in Chenopodium amaranticolor and C. quinoa, and systemic mosaic in Canavalia ensiformis and Phaseolus vulgaris cv. Jalo. However, the BCMV-Am isolate was not mechanically transmitted to Apium graveolens and Petroselinum crispum plants. To our knowledge, this is the first report of BCMV naturally infecting A. majus plants.

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蚕豆花叶病毒新天然寄主的研究

2023年，在巴西圣保罗州观察到两种出现花叶病症状的阿米马朱斯植物。通过透射电镜分析，发现叶提取物具有细长、弯曲的丝状颗粒，宽度为13 ~ 15 nm，长度为700 ~ 750 nm。此外，超薄切片分析显示细胞质圆柱形包涵体，这是Potyvirus属病毒的特征。使用通用引物、BCMV特异性引物和扩增子测序进行分子分析，证实存在大豆常见花叶病毒（BCMV, Potyvirus phaseovulgaris）。系统发育分析表明，该菌株（BCMV- am）与马兜铃属（Aristolochia spp.）植物BCMV分离株属一个分支。机械接种试验结果显示，苋菜藜和藜麦发生局部褪绿病变，菜豆和菜豆发生系统性花叶病。Jalo。然而，BCMV-Am分离株没有机械传播到石芹和石芹植株上。据我们所知，这是首次报道BCMV自然感染大马草。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Phytopathology 生物-植物科学

CiteScore

2.90

自引率

0.00%

发文量

审稿时长

4-8 weeks

期刊介绍： Journal of Phytopathology publishes original and review articles on all scientific aspects of applied phytopathology in agricultural and horticultural crops. Preference is given to contributions improving our understanding of the biotic and abiotic determinants of plant diseases, including epidemics and damage potential, as a basis for innovative disease management, modelling and forecasting. This includes practical aspects and the development of methods for disease diagnosis as well as infection bioassays. Studies at the population, organism, physiological, biochemical and molecular genetic level are welcome. The journal scope comprises the pathology and epidemiology of plant diseases caused by microbial pathogens, viruses and nematodes. Accepted papers should advance our conceptual knowledge of plant diseases, rather than presenting descriptive or screening data unrelated to phytopathological mechanisms or functions. Results from unrepeated experimental conditions or data with no or inappropriate statistical processing will not be considered. Authors are encouraged to look at past issues to ensure adherence to the standards of the journal.