{"title":"Identification of Novel Genes Involved in Chronological Life Span Extension in Saccharomyces cerevisiae","authors":"Bora Kim","doi":"10.1134/S0003683824607522","DOIUrl":null,"url":null,"abstract":"<p>The objectives of this study were to identify genes specifically associated with chronological life span extension and to elucidate the mechanisms of extension in <i>Saccharomyces</i> <i>cerevisiae</i>. In this study, two strains tolerant to oxidative stress and chronological life span extension were isolated through a transposon-mediated mutant library, and the disrupted genes were identified as <i>TDA10</i> and <i>TOH1</i>. In addition, the gene dosage of <i>TDA10</i> and <i>TOH1</i> was confirmed by qRT-PCR. In response to oxidative stress, mutant strains were found to reduce intracellular level of reactive oxygen species, increase expression of the <i>MSN2/4</i>, and induce the expression of cytosolic copper/zinc superoxide dismutase SOD1 and mitochondrial manganese superoxide dismutase SOD2.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"62 2","pages":"357 - 365"},"PeriodicalIF":1.1000,"publicationDate":"2026-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Applied Biochemistry and Microbiology","FirstCategoryId":"99","ListUrlMain":"https://link.springer.com/article/10.1134/S0003683824607522","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
The objectives of this study were to identify genes specifically associated with chronological life span extension and to elucidate the mechanisms of extension in Saccharomycescerevisiae. In this study, two strains tolerant to oxidative stress and chronological life span extension were isolated through a transposon-mediated mutant library, and the disrupted genes were identified as TDA10 and TOH1. In addition, the gene dosage of TDA10 and TOH1 was confirmed by qRT-PCR. In response to oxidative stress, mutant strains were found to reduce intracellular level of reactive oxygen species, increase expression of the MSN2/4, and induce the expression of cytosolic copper/zinc superoxide dismutase SOD1 and mitochondrial manganese superoxide dismutase SOD2.
期刊介绍：
Applied Biochemistry and Microbiology is an international peer reviewed journal that publishes original articles on biochemistry and microbiology that have or may have practical applications. The studies include: enzymes and mechanisms of enzymatic reactions, biosynthesis of low and high molecular physiologically active compounds; the studies of their structure and properties; biogenesis and pathways of their regulation; metabolism of producers of biologically active compounds, biocatalysis in organic synthesis, applied genetics of microorganisms, applied enzymology; protein and metabolic engineering, biochemical bases of phytoimmunity, applied aspects of biochemical and immunochemical analysis; biodegradation of xenobiotics; biosensors; biomedical research (without clinical studies). Along with experimental works, the journal publishes descriptions of novel research techniques and reviews on selected topics.
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