Sex-Dependent Effects of Rivastigmine on Melatonin Receptors in Middle-Aged Rats.

IF 1 4区医学 Q4 CLINICAL NEUROLOGY

Noropsikiyatri Arsivi-Archives of Neuropsychiatry Pub Date : 2026-03-26 eCollection Date: 2026-01-01 DOI:10.29399/npa.29283

Nilüfer Akgün Ünal, Elif Türkdönmez Ak, Ömer Ünal, Erdal Ağar, Mustafa Ayyıldız

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引用次数: 0

Abstract

Introduction: Melatonin (5-methoxy-N-acetyltryptamine) is closely linked to the body's natural circadian rhythm. Dementia is significantly more common among older individuals. Rivastigmine (RIVA) functions as a reversible inhibitor of acetylcholinesterase and butyrylcholinesterase, prescribed to help alleviate the symptoms of dementia. The mechanism behind RIVA's neuroendocrine interactions remains unclear despite its efficacy in treating specific symptoms. Our research aimed to investigate the effects of RIVA on memory, anxiety, and the expression of melatonin receptor genes MT1 and MT2 in middle-aged, 13-month-old male and female rats.

Methods: Rats were administered intraperitoneal injections of 2 mg/kg RIVA or saline once a day for ten days in succession. Twenty-four hours after the final injection, rats underwent the Y-maze and elevated plus maze tests, after which tissue samples were collected for qPCR analysis.

Results: Behavioral tests revealed sex-specific responses; elevated plus maze test results indicated that RIVA did not alter exploratory behavior in female rats but decreased it in male rats compared to controls. At the same time, RIVA treatment resulted in significant decreases of MT1 and MT2 gene expression in the hippocampus of both sexes, but cortical expression showed different patterns in males and system.

Conclusion: RIVA treatment in middle-aged rats may cause changes in MT1 and MT2 gene expression concomitant with behavioral alterations. The variation in melatonin receptor expression across sexes indicates a potential sex-dependent impact in aging populations, suggesting an interaction between cholinergic and melatonergic systems.

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利瓦斯汀对中年大鼠褪黑激素受体的性别依赖性影响。

褪黑素（5-甲氧基- n -乙酰色胺）与人体的自然昼夜节律密切相关。痴呆症在老年人中更为常见。利瓦斯汀明（RIVA）是一种可逆的乙酰胆碱酯酶和丁基胆碱酯酶抑制剂，用于帮助减轻痴呆症的症状。尽管RIVA在治疗特定症状方面有疗效，但其神经内分泌相互作用的机制尚不清楚。我们的研究旨在探讨RIVA对13月龄中年雄性和雌性大鼠的记忆、焦虑和褪黑激素受体基因MT1和MT2表达的影响。方法：大鼠腹腔注射RIVA 2 mg/kg或生理盐水，每天1次，连续10 d。最后一次注射24小时后，大鼠进行y形迷宫和升高正迷宫测试，之后收集组织样本进行qPCR分析。结果：行为测试揭示了性别特异性反应；升高的迷宫测试结果表明，与对照组相比，RIVA没有改变雌性大鼠的探索行为，但减少了雄性大鼠的探索行为。同时，RIVA处理导致两性海马MT1和MT2基因表达显著降低，但皮质表达在雄性和系统中表现出不同的模式。结论：RIVA治疗可引起中年大鼠MT1和MT2基因表达改变，并伴有行为改变。褪黑激素受体表达的性别差异表明，在老龄化人群中存在潜在的性别依赖性影响，表明胆碱能和褪黑激素系统之间存在相互作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Noropsikiyatri Arsivi-Archives of Neuropsychiatry CLINICAL NEUROLOGY-

CiteScore

1.70

自引率

9.10%

发文量

审稿时长

6-12 weeks

期刊介绍： Archives of Neuropsychiatry (Arch Neuropsychiatry) is the official journal of the Turkish Neuropsychiatric Society. It is published quarterly, and four editions annually constitute a volume. Archives of Neuropsychiatry is a peer reviewed scientific journal that publishes articles on psychiatry, neurology, and behavioural sciences. Both clinical and basic science contributions are welcomed. Submissions that address topics in the interface of neurology and psychiatry are encouraged. The content covers original research articles, reviews, letters to the editor, and case reports.