ITS phylogeny reveals a wide host range and a high genetic diversity of endophytic Phyllosticta capitalensis in Indonesia.

IF 1.4 4区生物学 Q4 MYCOLOGY

Mycoscience Pub Date : 2025-09-20 eCollection Date: 2025-01-01 DOI:10.47371/mycosci.2025.07.006

Indriati Ramadhani, Yoshiaki Tsuda, Hiroki Yamanobe, Kaoru Yamaguchi, Izumi Okane

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Abstract

Phyllosticta capitalensis is a cosmopolitan endophytic fungus with a wide host range. We hypothesize that endophytic P. capitalensis originates in tropical regions, which have higher host plant diversity than subtropical and temperate regions. We tested this hypothesis by examining the host range and genetic diversity in Indonesia, and the phylogeographic distribution of haplotypes according to the climatic conditions of Indonesia and elsewhere. A total of 434 plant species were sampled in the Bogor and Cibodas Botanical Gardens in Indonesia. A total of 244 ITS sequences of endophytic P. capitalensis from Indonesia and 133 previously collected from tropical, subtropical, and temperate regions of Japan were analyzed to assess genetic variation across climatic zones. Endophytic P. capitalensis was isolated from the leaves of 217 species from 182 genera in 35 families. The two Indonesian populations had higher haplotype diversities (16 and 11 haplotypes, respectively) than those at high-latitude sites. Assessment of haplotype phylogeny revealed a star-like pattern; Hap 2 was the dominant haplotype, being detected in 30 plant families in Indonesia, suggesting a rapid radiation of the species. The results demonstrate that P. capitalensis has higher genetic diversity in tropical than subtropical and temperate regions, supporting our hypothesis regarding its phylogenetic origin.

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ITS系统发育揭示了印尼内生毛竹的寄主范围广，遗传多样性高。

毛缕菌（Phyllosticta capitalensis）是一种寄主范围广泛的世界性内生真菌。我们推测，内生植物资本草起源于热带地区，热带地区的寄主植物多样性高于亚热带和温带地区。我们通过考察印度尼西亚的寄主范围和遗传多样性，以及根据印度尼西亚和其他地方的气候条件单倍型的系统地理分布来验证这一假设。在印度尼西亚茂物和奇博达斯植物园共采集了434种植物样本。本研究分析了来自印度尼西亚的244个内生植物的ITS序列，以及来自日本热带、亚热带和温带地区的133个ITS序列，以评估不同气候带的遗传变异。从35科182属217种植物的叶片中分离到一株内生真菌。两个印度尼西亚种群的单倍型多样性（分别为16和11个单倍型）高于高纬度地区。单倍型系统发育评估显示为星形模式；在印度尼西亚的30个植物科中检测到Hap 2为优势单倍型，表明该物种的快速辐射。结果表明，草芥在热带地区的遗传多样性高于亚热带和温带地区，支持了我们关于其系统发育起源的假设。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Mycoscience MYCOLOGY-

CiteScore

3.00

自引率

7.10%

发文量

审稿时长

3 months

期刊介绍： Mycoscience is the official English-language journal of the Mycological Society of Japan and is issued bimonthly. Mycoscience publishes original research articles and reviews on various topics related to fungi including yeasts and other organisms that have traditionally been studied by mycologists. The research areas covered by Mycoscience extend from such purely scientific fields as systematics, evolution, phylogeny, morphology, ecology, physiology, biochemistry, genetics, and molecular biology, to agricultural, medical, and industrial applications. New and improved applications of well-established mycological techniques and methods are also covered.