Homeobox transcription factor BmHox7 is required for sexual reproduction, but not for appressorial development and pathogenicity in Bipolaris maydis.

IF 1.4 4区生物学 Q4 MYCOLOGY

Mycoscience Pub Date : 2025-07-20 eCollection Date: 2025-01-01 DOI:10.47371/mycosci.2025.05.002

Seika Koya, Sae Shigeyoshi, Takuya Sumita, Toshikazu Irie, Kazumi Suzuki, Kosuke Izumitsu

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Abstract

Bipolaris maydis, the causal agent of southern corn leaf blight, possesses 10 putative homeobox genes in its genome. In this study, we disrupted and characterized a homeobox gene BmHox7 in B. maydis. While BmHox7 orthologues are required for appressorium formation in Sordariomycetous plant pathogenic fungi (Magnaporthe oryzae, Colletotrichum orbiculare, and C. scovillei), ΔBmHox7 strains formed appressoria normally and exhibited virulence comparable to the wild-type strain. However, in crossing test, ΔBmHox7 strains produced small, immature pseudothecia lacking beaks. Most pseudothecia of BmHox7 mutants contained no asci, indicating that BmHox7 is crucial for sexual reproduction. These findings demonstrate that in B. maydis, BmHox7 is crucial for sexual reproduction but dispensable for appressorial formation and pathogenicity.

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同源盒转录因子BmHox7是雌雄同体繁殖所必需的，但不是雌雄同体发育和致病性的必需因子。

南方玉米叶枯病的致病因子双斑螟（Bipolaris maydis）在其基因组中具有10个假定的同源盒基因。在这项研究中，我们破坏了maydis的同源盒基因BmHox7并对其进行了表征。虽然sordariomycous植物病原真菌（Magnaporthe oryzae， Colletotrichum orbiculare和C. scovillei）需要BmHox7同源物形成附着胞，但ΔBmHox7菌株正常形成附着胞，并表现出与野生型菌株相当的毒力。然而，在杂交试验中，ΔBmHox7菌株产生了小的，未成熟的假毛虫，没有喙。大多数BmHox7突变体假胚不含asci，表明BmHox7对有性生殖至关重要。这些发现表明，BmHox7对maydis的有性繁殖至关重要，但对附着子的形成和致病性却没有作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Mycoscience MYCOLOGY-

CiteScore

3.00

自引率

7.10%

发文量

审稿时长

3 months

期刊介绍： Mycoscience is the official English-language journal of the Mycological Society of Japan and is issued bimonthly. Mycoscience publishes original research articles and reviews on various topics related to fungi including yeasts and other organisms that have traditionally been studied by mycologists. The research areas covered by Mycoscience extend from such purely scientific fields as systematics, evolution, phylogeny, morphology, ecology, physiology, biochemistry, genetics, and molecular biology, to agricultural, medical, and industrial applications. New and improved applications of well-established mycological techniques and methods are also covered.