High frequency of Streptococcus pneumoniae serotype 10A identified by PCR in cerebrospinal fluid culture-negative paediatric clinical samples

IF 2.2 4区医学 Q3 INFECTIOUS DISEASES

Enfermedades infecciosas y microbiologia clinica Pub Date : 2026-04-01 Epub Date: 2026-01-22 DOI:10.1016/j.eimc.2025.503070

Juan Carlos Sanz , Marta Pérez-Abeledo , Ana Garrido-Buenache , Susana Jiménez , Fernando Martín , Jesús Iñigo

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Abstract

Introduction

The aim of this study was to determine the distribution of Streptococcus pneumoniae (SP) serotypes in cerebrospinal fluid (CSF) samples, according to the microbiological detection strategy.

Methods

Pneumococcal strains identification and serotyping were performed by conventional CSF culture and Quellung serotyping (CI-CS). In CSF culture-negative samples, identification and serotyping were conducted using direct PCR serotyping (PCR-DS).

Results

Between 2018 and 2024, 144 strains/CSF samples (31 children and 113 adult cases) were studied. Serotypes 3, 8, 10A, 23B, 12B/12F and 6C accounted for the 54.9%. Serotype 10A represented 36.4% of those detected by PCR-DS in culture-negative CSF samples (4/11 cases [4/8 paediatric]) versus 6.0% detected by CI-CS (8/133 cases [3/23 paediatric]; p < 0.01).

Conclusions

Direct PCR detection and serotyping in CSF clinical samples can complement the culture-Quellung serotyping and enhance the diagnosis of paediatric pneumococcal meningitis caused by serotype 10A.

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脑脊液培养阴性儿童临床标本中10A型肺炎链球菌的PCR检测频率较高

本研究的目的是根据微生物检测策略确定脑脊液（CSF）样本中肺炎链球菌（SP）血清型的分布。方法采用常规脑脊液培养和Quellung血清分型（CI-CS）对肺炎球菌进行菌株鉴定和血清分型。对脑脊液培养阴性样本，采用直接PCR血清分型（PCR- ds）进行鉴定和血清分型。结果2018 - 2024年共收集144株/CSF样本，其中儿童31例，成人113例。血清型3、8、10A、23B、12B/12F和6C占54.9%。培养阴性脑脊液样本中，PCR-DS检测到10A血清型的占36.4%(4/11例[4/8儿科])，CI-CS检测到6.0%（8/133例[3/23儿科]；p < 0.01）。结论脑脊液临床标本的直接PCR检测和血清分型可与培养- quellung血清分型相补充，提高对10A型儿童肺炎球菌脑膜炎的诊断。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Enfermedades infecciosas y microbiologia clinica 医学-传染病学

CiteScore

2.10

自引率

8.00%

发文量

194

审稿时长

29 days

期刊介绍： Hoy está universalmente reconocida la renovada y creciente importancia de la patología infecciosa: aparición de nuevos agentes patógenos, de cepas resistentes, de procesos con expresión clínica hasta ahora desconocida, de cuadros de una gran complejidad. Paralelamente, la Microbiología y la Infectología Clínicas han experimentado un gran desarrollo como respuesta al reto planteado por la actual patología infecciosa. Enfermedades Infecciosas y Microbiología Clínica es la Publicación Oficial de la Sociedad Española SEIMC. Cumple con la garantía científica de esta Sociedad, la doble función de difundir trabajos de investigación, tanto clínicos como microbiológicos, referidos a la patología infecciosa, y contribuye a la formación continuada de los interesados en aquella patología mediante artículos orientados a ese fin y elaborados por autores de la mayor calificación invitados por la revista.