Effect of bupivacaine 0.25% and 0.5% on nociceptive, motor and proprioceptive function in an ultrasound-guided sciatic nerve block model in rats

IF 1.9 2区农林科学 Q2 VETERINARY SCIENCES

Veterinary anaesthesia and analgesia Pub Date : 2026-05-01 Epub Date: 2026-02-06 DOI:10.1016/j.vaa.2026.101201

Hedie A. Bustamante, Douglas Castro, Erik H. Hofmeister, Stuart Clark-Price

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引用次数: 0

Abstract

Objective

To evaluate the nociceptive, motor and proprioceptive response after ultrasound-guided perineural sciatic nerve injections of bupivacaine 0.25% and 0.5% in Wistar rats.

Study design

Prospective, randomized, blinded crossover study.

Animals

A group of eight healthy adult female Wistar rats.

Methods

Rats underwent isoflurane anesthesia and were given ultrasound-guided sciatic nerve injections of 0.1 mL of 0.25% or 0.5% bupivacaine, with a 1 week recovery period between injections. Nociceptive function [electronic von Frey (grams, response)], motor function [grip test, gait response (1–3 scale)] and proprioceptive function [tactile placing (0–3 scale) and paw positioning (clubbed digits/normal splaying)] were evaluated at baseline and at 5, 10, 20, 30, 40, 60, 90, 120, 180, 240, 300 and 360 minutes after anesthesia. Data were analyzed using mixed models with significance set at p < 0.05.

Results

Sciatic nerve blockade was effective from 20 minutes into recovery, with treatment, time and interaction on nociceptive function being significant from 20 to 240 minutes (p < 0.05). In Group 0.25%, sensory function peaked at 60 minutes [111.2 g, 95% confidence interval (CI) 100.8–121.5] and returned to a baseline response at 240 minutes (55.5 g, 95% CI: 45.2–65.9). Significant differences between treatment groups were observed from 5 to 240 minutes. Motor and proprioceptive functions were reduced at 20 minutes [median score: 1, interquartile range (IQR: 1)] and returned to baseline at 240 minutes. In Group 0.5%, sensory function peaked at 90 minutes (133.7 g, 95% CI: 123.3–144.1) and returned to baseline (52.0 g, 95% CI: 41.6–62.4) in 300 minutes. Motor and proprioceptive functions were reduced at 10 minutes [1 (IQR: 0.25)] and returned to baseline by 300 minutes.

Conclusions and clinical relevance

Both 0.25% and 0.5% bupivacaine concentrations produced effective sciatic nerve blockade in all rats, demonstrating a concentration-dependent effect on the duration and magnitude of nociceptive, motor and proprioceptive block.

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0.25%和0.5%布比卡因对超声引导坐骨神经阻滞模型大鼠伤害、运动和本体感觉功能的影响。

目的：观察超声引导下坐骨神经周围注射0.25%和0.5%布比卡因对Wistar大鼠损伤性、运动性和本体性的影响。研究设计：前瞻性、随机、盲法交叉研究。动物：一组8只健康成年雌性Wistar大鼠。方法：异氟醚麻醉大鼠，超声引导下坐骨神经注射0.25%或0.5%布比卡因0.1 mL，两次注射恢复期为1周。在基线和麻醉后5、10、20、30、40、60、90、120、180、240、300和360分钟评估伤害感觉功能[电子von Frey（克，反应）]、运动功能[握力测试、步态反应（1-3分）]和本体感觉功能[触觉放置（0-3分）和爪定位（杵状指/正常张开）]。数据分析采用混合模型，显著性设置为p < 0.05。结果：坐骨神经阻滞在恢复20 min时有效，20 ~ 240 min时治疗、时间及相互作用对伤害功能的影响均显著（p < 0.05）。在0.25%组，感觉功能在60分钟达到峰值[111.2 g， 95%可信区间（CI） 100.8-121.5]，并在240分钟（55.5 g, 95% CI: 45.2-65.9）恢复到基线反应。从5分钟到240分钟观察到治疗组之间的显著差异。运动和本体感觉功能在20分钟时降低[中位数得分：1，四分位间距（IQR: 1）]，并在240分钟时恢复到基线。在0.5%组，感觉功能在90分钟达到峰值（133.7 g, 95% CI: 123.3-144.1），在300分钟恢复到基线（52.0 g, 95% CI: 41.6-62.4）。运动和本体感觉功能在10分钟时降低[1 (IQR: 0.25)]， 300分钟时恢复到基线。结论和临床意义：0.25%和0.5%布比卡因浓度对所有大鼠均产生有效的坐骨神经阻断作用，对伤害感觉、运动和本体感觉阻断的持续时间和程度具有浓度依赖性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Veterinary anaesthesia and analgesia 农林科学-兽医学

CiteScore

3.10

自引率

17.60%

发文量

审稿时长

97 days

期刊介绍： Veterinary Anaesthesia and Analgesia is the official journal of the Association of Veterinary Anaesthetists, the American College of Veterinary Anesthesia and Analgesia and the European College of Veterinary Anaesthesia and Analgesia. Its purpose is the publication of original, peer reviewed articles covering all branches of anaesthesia and the relief of pain in animals. Articles concerned with the following subjects related to anaesthesia and analgesia are also welcome: the basic sciences; pathophysiology of disease as it relates to anaesthetic management equipment intensive care chemical restraint of animals including laboratory animals, wildlife and exotic animals welfare issues associated with pain and distress education in veterinary anaesthesia and analgesia. Review articles, special articles, and historical notes will also be published, along with editorials, case reports in the form of letters to the editor, and book reviews. There is also an active correspondence section.