Anti-Proliferative Effects of Resveratrol on Gingival Fibroblasts Derived From Amlodipine-Induced Gingival Overgrowth

IF 5.4 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of periodontal research Pub Date : 2026-08-13 Epub Date: 2026-04-11 DOI:10.1111/jre.70112

Bilkan Kara, Nuriye Işıl Saygun, Cansel Köse Özkan, Melis Özgül Slezovic, Muhittin Abdulkadir Serdar, Mualla Pınar Elçi, Alpdoğan Kantarci

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引用次数: 0

Abstract

Aim

This study aimed to investigate the anti-proliferative effects of resveratrol on gingival fibroblasts derived from amlodipine-induced gingival overgrowths and its impact on the levels of transforming growth factor-β1 (TGF-β1), connective tissue growth factor (CTGF), collagen type I (COL-1), interleukin-6 (IL-6), transglutaminase-2 (TGM-2), and superoxide dismutase (SOD), which play critical roles in collagen and extracellular matrix metabolism in gingival enlargement.

Methods

Primary gingival fibroblasts were isolated from patients with amlodipine-induced gingival overgrowth (GO group) and from healthy gingival tissues (H group). Each group was divided into untreated and resveratrol-treated subgroups. Cell proliferation and viability at 24 and 48 h were assessed by MTT assay. The levels of TGF-β1, CTGF, COL-1, IL-6, TGM-2, and SOD were quantified using ELISA.

Results

MTT values were significantly higher in the GO group than in the H group at both 24 and 48 h (p = 0.01). Resveratrol treatment significantly decreased proliferation in the GO group at both time points (p < 0.001). In the GO group, resveratrol reduced TGF-β1 (p = 0.0090), CTGF (p = 0.0090), TGM-2 (p = 0.0088), COL-1 (p = 0.0139), and IL-6 (p = 0.050) levels at 24 h and significantly increased SOD levels (p = 0.0143).

Conclusion

Resveratrol suppressed cellular proliferation without exerting cytotoxic effects in amlodipine-induced GO, while downregulating fibrosis markers (TGF-β1, CTGF, COL-1, IL-6, and TGM-2) and restoring diminished SOD synthesis.

Trial Registiration

This study did not involve a clinical trial and therefore registration was not required

查看原文本刊更多论文

白藜芦醇对氨氯地平诱导牙龈过度生长的成纤维细胞的抗增殖作用。

目的：本研究旨在探讨白藜芦醇对氨氯地平诱导的牙龈过度生长的成纤维细胞的抗增殖作用及其对牙龈增生过程中胶原和细胞外基质代谢中起关键作用的转化生长因子-β1 （TGF-β1）、结缔组织生长因子（CTGF）、I型胶原（COL-1）、白细胞介素-6 （IL-6）、转谷氨酰胺酶-2 （TGM-2）、超氧化物歧化酶（SOD）水平的影响。方法：分别从氨氯地平诱导的牙龈过度生长患者（GO组）和健康牙龈组织（H组）中分离原代牙龈成纤维细胞。各组分为未治疗组和白藜芦醇治疗组。MTT法测定24、48 h细胞增殖和活力。ELISA法测定TGF-β1、CTGF、COL-1、IL-6、TGF- 2、SOD水平。结果：在24和48 H，氧化石墨烯组的MTT值均显著高于H组（p = 0.01）。结论：白藜芦醇抑制氨氯地平诱导的氧化石墨烯细胞增殖，但不产生细胞毒性作用，同时下调纤维化标志物（TGF-β1、CTGF、COL-1、IL-6和TGF- 2），恢复减少的SOD合成。试验注册：本研究不涉及临床试验，因此不需要注册。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of periodontal research 医学-牙科与口腔外科

CiteScore

6.90

自引率

5.70%

发文量

103

审稿时长

6-12 weeks

期刊介绍： The Journal of Periodontal Research is an international research periodical the purpose of which is to publish original clinical and basic investigations and review articles concerned with every aspect of periodontology and related sciences. Brief communications (1-3 journal pages) are also accepted and a special effort is made to ensure their rapid publication. Reports of scientific meetings in periodontology and related fields are also published. One volume of six issues is published annually.