[Advances in the construction and application of a novel liver organoid model for hepatitis B virus infection].

Abstract

Hepatitis B virus (HBV) infection is one of the leading causes of liver cirrhosis and hepatocellular carcinoma, posing a threat to human health, yet its specific pathogenic mechanisms have not been fully elucidated. Organoid models have shown great potential in recent years due to significant limitations in existing cellular and animal models for HBV research. The construction of in vitro liver organoid models is important for better exploring HBV pathogenic mechanisms and achieving early-stage prevention and treatment. Currently, human liver organoid (HLO) models have been explored using various differentiation and culture protocols and have been confirmed to hold enormous potential in tissue development, disease model construction, personalized medicine, drug screening, and other aspects. This article briefly outlines the characteristics, differentiations, culture strategies, and applications of the emerging HLO model, thus providing a reference value for better understanding and optimizing support to HBV research.