Association of Helicobacter pylori with Candida albicans enhances fungal virulence and stress tolerance.

IF 4 3区医学 Q1 GASTROENTEROLOGY & HEPATOLOGY

Gut Pathogens Pub Date : 2026-04-09 DOI:10.1186/s13099-026-00831-7

Jianchao Sun, Qing Luo, Tingxiu Yang, Xiaoli Xu, Tingting Luo, Huifeng Jian, Xianli Chen, Guzhen Cui, Zhenghong Chen

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Abstract

Background: Bacterial associations with fungal hosts are increasingly recognized as common rather than exceptional. Among these interactions, Candida spp. and Helicobacter pylori are of particular interest, as evidence suggests that Candida may serve as a potential host for H. pylori, facilitating its persistence and dissemination. Although interactions between Candida spp. and members of the bacterial microbiota-particularly H. pylori-are increasingly recognized for their role in modulating microbial ecology and influencing pathological outcomes in the host, the impact of H. pylori on Candida albicans remains poorly characterized. Whether this interaction alters the biological behavior or pathogenic potential of Candida spp. remains poorly understood. In this study, C. albicans strains CacoHp, which were detected to be positive for H. pylori-specific genes, were generated through co-culture, and the effects of this bacterial-fungal interaction on tolerance and virulence were investigated.

Results: Co-culture with H. pylori S7 and C. albicans SC5314 yielded Hp-positive C. albicans CacoHp. Compared with the parental strain SC5314, CacoHp exhibited increased tolerance to antifungal agents, sodium dodecyl sulfate, and H₂O₂; enhanced inhibition of GES-1 cell proliferation; elevated aspartic protease secretion; and increased hyphal formation. Proteomic and quantitative polymerase chain reaction analyses indicated upregulation of ergosterol transport (SNQ2) and vacuolar ATPase (VMA8) pathways, and enhanced stress tolerance. In mice, CacoHp induced a stronger inflammatory response and more severe gastric tissue damage than the SC5314 strain.

Conclusions: Co-culture with H. pylori generates C. albicans strains CacoHp, which were detected to be positive for H. pylori-specific genes, that exhibit enhanced chemical stress tolerance and increased virulence. This study first revealed the influence of H. pylori on Candida phenotypes, further supporting the clinical relevance of their interaction.

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幽门螺杆菌与白色念珠菌的关联增强了真菌毒力和应激耐受性。

背景：细菌与真菌宿主的关联越来越被认为是普遍而非例外。在这些相互作用中，念珠菌和幽门螺杆菌尤其令人感兴趣，因为有证据表明念珠菌可能是幽门螺杆菌的潜在宿主，促进其持续存在和传播。尽管念珠菌与细菌微生物群成员（尤其是幽门螺杆菌）之间的相互作用在调节微生物生态和影响宿主病理结果方面的作用越来越被认识到，但幽门螺杆菌对白色念珠菌的影响仍然知之甚少。这种相互作用是否会改变念珠菌的生物学行为或致病潜力仍然知之甚少。本研究通过共培养产生了检出幽门螺杆菌特异性基因阳性的白色念珠菌CacoHp菌株，并研究了这种细菌-真菌相互作用对耐受性和毒力的影响。结果：幽门螺旋杆菌S7和白色念珠菌SC5314共培养产生hp阳性的白色念珠菌CacoHp。与亲本菌株SC5314相比，CacoHp对抗真菌药物、十二烷基硫酸钠和H2O2的耐受性增强；增强抑制GES-1细胞增殖；天冬氨酸蛋白酶分泌升高；菌丝形成增多。蛋白质组学和定量聚合酶链反应分析表明，麦角甾醇转运（SNQ2）和液泡atp酶（VMA8）途径上调，并增强了胁迫耐受性。在小鼠中，与SC5314菌株相比，CacoHp诱导了更强的炎症反应和更严重的胃组织损伤。结论：与幽门螺杆菌共培养产生白色念珠菌CacoHp，该菌株检测到幽门螺杆菌特异性基因阳性，表现出增强的化学应激耐受性和增强的毒力。这项研究首次揭示了幽门螺杆菌对念珠菌表型的影响，进一步支持了它们相互作用的临床相关性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Gut Pathogens GASTROENTEROLOGY & HEPATOLOGY-MICROBIOLOGY

CiteScore

7.70

自引率

2.40%

发文量

期刊介绍： Gut Pathogens is a fast publishing, inclusive and prominent international journal which recognizes the need for a publishing platform uniquely tailored to reflect the full breadth of research in the biology and medicine of pathogens, commensals and functional microbiota of the gut. The journal publishes basic, clinical and cutting-edge research on all aspects of the above mentioned organisms including probiotic bacteria and yeasts and their products. The scope also covers the related ecology, molecular genetics, physiology and epidemiology of these microbes. The journal actively invites timely reports on the novel aspects of genomics, metagenomics, microbiota profiling and systems biology. Gut Pathogens will also consider, at the discretion of the editors, descriptive studies identifying a new genome sequence of a gut microbe or a series of related microbes (such as those obtained from new hosts, niches, settings, outbreaks and epidemics) and those obtained from single or multiple hosts at one or different time points (chronological evolution).