Application of wood sawdust microbial transformation for the utilization of long-term stored bark-wood waste.

IF 2.1 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Ludmila Aleksandrovna Belovezhets, Dmitry Sergeevich Belousov, Nadezhda Vladimirovna Filinova, Ekaterina Olegovna Pristavka, Aleksey Aleksandrovich Pristavka
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Abstract

The issue of bark-wood waste (BWW) utilization is challenging for the regions adjacent to pulp and paper mills. Bark is difficult to recycle, and the additives used in paper production further complicate this process. This work focuses on the potential application of wood sawdust microbial transformation, the technology of which was developed at the Irkutsk Institute of Chemistry SB RAS, for the processing of bark-wood waste into environmentally safe soil. Laboratory experiments revealed that long-term stored BWW can undergo microbial degradation. The best results were obtained with the BWW/sawdust mixture. The substrate neutralization conditions were selected to simultaneously lower the pH and introduce the necessary mineral additives for microbial function. A semi-industrial experiment with a BWW/sawdust mixture of 20 m3 (3:1 ratio) was performed. The experiment demonstrated that pH stabilization occurred more efficiently than in the laboratory experiment. A temperature increase, which is characteristic of aerobic processing of lignocellulosic waste was also observed. The product obtained was non-toxic to both plants and Chlorella vulgaris (hazard class 5). The processing improved of the agrochemical parameters of the substrate, such as hydrolytic acidity, cation exchange capacity, and the sum of absorbed bases. The increase in humic acid content indicates a deep microbial degradation of the lignin component of BWW. Consequently, the microbial transformation of BWW was successfully implemented to produce a non-toxic soil. This technology can be scaled up for large volumes of BWW at storage sites.

木屑微生物转化在长期贮存树皮木废弃物利用中的应用。
树皮木废料(BWW)的利用问题对纸浆和造纸厂附近的地区具有挑战性。树皮很难回收,造纸中使用的添加剂进一步使这一过程复杂化。这项工作的重点是木屑微生物转化的潜在应用,该技术是由伊尔库茨克化学研究所SB RAS开发的,用于将树皮木材废料加工成环境安全的土壤。实验室实验表明,长期储存的BWW可发生微生物降解。BWW/锯末混合物的效果最好。选择底物中和条件,同时降低pH值并引入微生物功能所需的矿物添加剂。以20 m3(3:1)的生物质/木屑混合物进行了半工业试验。实验表明,pH稳定比实验室实验更有效。还观察到温度升高,这是好氧处理木质纤维素废物的特征。所得产物对植物和普通小球藻(危害5级)均无毒。该处理改善了基质的农化参数,如水解酸度、阳离子交换能力和吸收碱的总量。腐植酸含量的增加表明木质素成分被微生物深度降解。因此,BWW的微生物转化成功地实现了生产无毒土壤。这项技术可以扩展到储存地点的大量生物垃圾。
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来源期刊
Biotechnology Letters
Biotechnology Letters 工程技术-生物工程与应用微生物
CiteScore
5.90
自引率
3.70%
发文量
108
审稿时长
1.2 months
期刊介绍: Biotechnology Letters is the world’s leading rapid-publication primary journal dedicated to biotechnology as a whole – that is to topics relating to actual or potential applications of biological reactions affected by microbial, plant or animal cells and biocatalysts derived from them. All relevant aspects of molecular biology, genetics and cell biochemistry, of process and reactor design, of pre- and post-treatment steps, and of manufacturing or service operations are therefore included. Contributions from industrial and academic laboratories are equally welcome. We also welcome contributions covering biotechnological aspects of regenerative medicine and biomaterials and also cancer biotechnology. Criteria for the acceptance of papers relate to our aim of publishing useful and informative results that will be of value to other workers in related fields. The emphasis is very much on novelty and immediacy in order to justify rapid publication of authors’ results. It should be noted, however, that we do not normally publish papers (but this is not absolute) that deal with unidentified consortia of microorganisms (e.g. as in activated sludge) as these results may not be easily reproducible in other laboratories. Papers describing the isolation and identification of microorganisms are not regarded as appropriate but such information can be appended as supporting information to a paper. Papers dealing with simple process development are usually considered to lack sufficient novelty or interest to warrant publication.
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