Impact of EDTA Formulations and Activation Methods on Stem Cell Response, Antimicrobial Activity and Dentine Integrity: An In Vitro Study

IF 1.7 4区医学 Q3 DENTISTRY, ORAL SURGERY & MEDICINE

Australian Endodontic Journal Pub Date : 2026-08-04 Epub Date: 2026-04-08 DOI:10.1111/aej.70076

Letycia Accioly Simões Coelho, Laura Ferreira Almeida, Ester Alves Ferreira Bordini, Fernanda Balestrero Cassiano, Mirela Cesar Barros, Talita Tartari, Flaviana Bombarda de Andrade, Diana Gabriela Soares dos Passos, Marco Antonio Hungaro Duarte

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Abstract

This study evaluated the effects of 17% trisodium EDTA and 15% disodium EDTA (EDTAd), with or without activation, on SCAP response, antimicrobial activity and dentine chemical alterations. SCAP viability (Live/Dead) and metabolic activity (Alamar Blue) were assessed, along with antibiofilm and intratubular disinfection against Enterococcus faecalis and Streptococcus mutans, after irrigation with EDTA or EDTAd delivered by conventional irrigation (CI), ultrasonic activation (UA) or diode laser activation (LA). Dentine alterations were analysed by Fourier-transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). EDTA preserved higher SCAP metabolic activity, particularly at early time points (p < 0.05), while cytotoxicity did not differ among groups. Antibiofilm and intratubular antimicrobial effects were similar across irrigants and activation methods (p > 0.05). EDTAd caused greater alterations in the amide III/phosphate ratio (p < 0.05), with increased smear-layer removal and surface irregularities. EDTA showed greater biological compatibility and fewer dentine alterations, whereas EDTAd induced more pronounced structural changes without antimicrobial differences.

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EDTA配方和激活方法对干细胞反应、抗菌活性和牙本质完整性的影响：一项体外研究

本研究评估了17%三钠EDTA和15%二钠EDTA （EDTAd）在活化或未活化下对SCAP反应、抗菌活性和牙本质化学改变的影响。通过常规灌洗（CI）、超声激活（UA）或二极管激光激活（LA）给EDTA或EDTAd灌洗后，评估SCAP活力（活/死）和代谢活性（Alamar Blue），以及抗生素膜和小管内对粪肠球菌和变形链球菌的消毒。采用傅里叶变换红外光谱（FTIR）和扫描电镜（SEM）分析牙本质变化。EDTA保留了较高的SCAP代谢活性，特别是在早期时间点（p 0.05）。EDTAd引起了更大的酰胺III/磷酸盐比率的改变(p

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来源期刊

Australian Endodontic Journal DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

3.50

自引率

6.20%

发文量

审稿时长

>12 weeks

期刊介绍： The Australian Endodontic Journal provides a forum for communication in the different fields that encompass endodontics for all specialists and dentists with an interest in the morphology, physiology, and pathology of the human tooth, in particular the dental pulp, root and peri-radicular tissues. The Journal features regular clinical updates, research reports and case reports from authors worldwide, and also publishes meeting abstracts, society news and historical endodontic glimpses. The Australian Endodontic Journal is a publication for dentists in general and specialist practice devoted solely to endodontics. It aims to promote communication in the different fields that encompass endodontics for those dentists who have a special interest in endodontics.