High-quality genome assembly of Aglaia odorata reveals evolution, terpenes diversity and abundance in Meliaceae.

IF 10 Q1 HORTICULTURE

Molecular Horticulture Pub Date : 2026-04-09 DOI:10.1186/s43897-025-00212-9

Zhiyu Chen, Xingyu Yang, Tianyu Yang, Xin Yin, Danni Yang, Xuefei Yang, Yunqiang Yang, Yongping Yang

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引用次数: 0

Abstract

Meliaceae species are known for producing diverse metabolites, yet the role of whole-genome duplication (WGD) in shaping this diversity remains unclear. In this study, we analyzed volatile metabolites in Azadirachta indica, Toona ciliata and Aglaia odorata, finding terpenes to be most abundant in A. odorata. A high-quality genome assembly of A. odorata revealed lineage-specific whole-genome triplication (WGT), the first reported in Meliaceae. Comparative genomics indicated extensive chromosomal rearrangements, biased gene retention, gene expression, and long terminal repeat (LTR) insertions during the evolution of subgenomes in Meliaceae, with A. odorata showing subgenome dominance. Despite a reduction in terpene synthase (TPS) gene copies, the specifically highly expression of AodTPS25, AodTPS21, AodTPS24 and AodTPS26 in A. odorata could be crucial for its terpene abundance and diversity through transcriptomics and enzyme assays. Moreover, the ethylene response factor AodERF27 was found to activate AodTPS26, linking transcriptional regulation to terpene diversity. These findings uncovered the genomic and molecular mechanisms in Meliaceae.

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高质量的香缕草基因组组装揭示了香缕草科植物萜类的进化、多样性和丰度。

众所周知，Meliaceae物种以产生多种代谢物而闻名，但全基因组复制（WGD）在形成这种多样性中的作用尚不清楚。在本研究中，我们分析了印楝、香椿和香椿的挥发性代谢物，发现香椿中萜烯含量最高。一个高质量的基因组组装揭示了谱系特异性全基因组三倍扩增（WGT），首次在甜叶科报道。比较基因组学研究表明，在梅兰科植物亚基因组的进化过程中，存在着广泛的染色体重排、偏倚的基因保留、基因表达和长末端重复序列（LTR）插入等现象，其中，气味木香属植物表现出亚基因组优势。尽管萜烯合成酶（TPS）基因拷贝数减少，但通过转录组学和酶学分析，AodTPS25、AodTPS21、AodTPS24和AodTPS26在臭椿中的高表达可能对其萜烯丰度和多样性至关重要。此外，乙烯响应因子AodERF27被发现激活AodTPS26，将转录调控与萜烯多样性联系起来。这些发现揭示了楝科植物的基因组和分子机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular Horticulture horticultural research-

CiteScore

8.00

自引率

0.00%

发文量

审稿时长

12 weeks

期刊介绍： Aims Molecular Horticulture aims to publish research and review articles that significantly advance our knowledge in understanding how the horticultural crops or their parts operate mechanistically. Articles should have profound impacts not only in terms of high citation number or the like, but more importantly on the direction of the horticultural research field. Scope Molecular Horticulture publishes original Research Articles, Letters, and Reviews on novel discoveries on the following, but not limited to, aspects of horticultural plants (including medicinal plants): ▪ Developmental and evolutionary biology ▪ Physiology, biochemistry and cell biology ▪ Plant-microbe and plant-environment interactions ▪ Genetics and epigenetics ▪ Molecular breeding and biotechnology ▪ Secondary metabolism and synthetic biology ▪ Multi-omics dealing with data sets of genome, transcriptome, proteome, metabolome, epigenome and/or microbiome. The journal also welcomes research articles using model plants that reveal mechanisms and/or principles readily applicable to horticultural plants, translational research articles involving application of basic knowledge (including those of model plants) to the horticultural crops, novel Methods and Resources of broad interest. In addition, the journal publishes Editorial, News and View, and Commentary and Perspective on current, significant events and topics in global horticultural fields with international interests.