Aging effects on emotionality, cognition and brain mononuclear cells in Sprague-Dawley rats of both sexes.

IF 6 Q2 GERIATRICS & GERONTOLOGY

npj aging Pub Date : 2026-04-07 DOI:10.1038/s41514-026-00364-y

Jasmina Djuretić, Jana Ivanović, Kristina Jezdić, Vanja Todorović, Biljana Bufan, Anja Santrač, Jovana Aranđelović Ilić

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Abstract

Aging impairs the function of immune cells and increases susceptibility to diseases such as anxiety and dementia. Nevertheless, some individuals exhibit resilience to age-related impairments, although the mechanisms are still unclear. This study investigated alterations in brain mononuclear cells and their association with age-related behavioral deficits in male and female rats. Flow cytometry was used to analyze the expression of CD45, CX3CR1, and CD163 on brain CD11b+ cells. Aging rats demonstrated reduced anxiety-like behavior, spatial learning, and social play, with certain sex differences. Young adult females showed hyperactivity and higher cognitive flexibility than same-aged males. Aging increased CD45 and CD163 expression within CD11b+ cells. Furthermore, sex-dependent correlations were observed between expression of CD163 and CX3CR1 within CD11b+ cells and locomotor activity in aging. Females might appear to be more susceptible to aging, suggesting microglial activation as a compensatory mechanism. These results suggest immune cell dynamics underlying sex-specific aging behavior.

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衰老对Sprague-Dawley大鼠情绪、认知和脑单核细胞的影响。

衰老会损害免疫细胞的功能，增加对焦虑和痴呆等疾病的易感性。尽管如此，一些人对年龄相关的损伤表现出弹性，尽管机制尚不清楚。本研究调查了雄性和雌性大鼠脑单核细胞的变化及其与年龄相关行为缺陷的关系。流式细胞术分析CD45、CX3CR1和CD163在脑CD11b+细胞上的表达。衰老大鼠表现出焦虑样行为、空间学习和社交游戏的减少，并存在一定的性别差异。年轻成年女性表现出多动症和比同龄男性更高的认知灵活性。衰老增加了CD11b+细胞中CD45和CD163的表达。此外，CD11b+细胞中CD163和CX3CR1的表达与衰老过程中运动活性之间存在性别依赖的相关性。女性似乎更容易衰老，这表明小胶质细胞的激活是一种补偿机制。这些结果表明免疫细胞动力学是性别特异性衰老行为的基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

npj aging

CiteScore

8.90

自引率

0.00%

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