Biomedical applications of green-synthesized selenium and iron-selenium bimetallic nanoflowers using Azadirachta indica leaf extract.

IF 4.4 4区生物学 Q1 BIOCHEMICAL RESEARCH METHODS

IEEE Transactions on NanoBioscience Pub Date : 2026-04-06 DOI:10.1109/TNB.2026.3681130

Vishal Chikhale, Gajanan Sonawane, Mamata Singhvi, Beom Soo Kim

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Abstract

Green synthesis of nanoparticles (NPs) has gained significant attention due to its environmentally friendly approach and potential biomedical applications. This study focuses on the synthesis of selenium nanoparticles (SeNPs) and iron-selenium bimetallic nanoparticles (Fe-SeNPs) using Azadirachta indica leaf extract as a natural reducing and stabilizing agent. The synthesized nanoparticles were characterized using UV-Vis spectroscopy, DLS, FTIR, XRD, SEM, TEM, and TGA, confirming their successful synthesis. The therapeutic efficacy of SeNPs and Fe-SeNPs was evaluated against MCF-7 human breast cancer cells through MTT, wound healing, apoptosis, and yolk sac membrane (YSM) assays. Fe-SeNPs demonstrated greater cytotoxicity than SeNPs, with IC50 values of 50 μg/mL at 24 h and 30 μg/mL at 48 h. Both types of nanoparticles significantly inhibited cell migration (56.6% for SeNPs and 61.4% for Fe-SeNPs at 48 h) and promoted apoptosis, as confirmed by Annexin V/PI staining. Further, a dose-dependent inhibition of angiogenesis was observed in the YSM assay, with complete inhibition at higher concentrations. These results highlight the potential of green-synthesized SeNPs and Fe-SeNPs as promising candidates for breast cancer treatment by inducing cytotoxicity, suppressing migration and angiogenesis, and promoting apoptosis, thereby contributing to the advancement of nanoparticle-based cancer therapeutics.

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印楝叶提取物绿色合成硒和铁硒双金属纳米花的生物医学应用。

纳米颗粒的绿色合成因其环境友好性和潜在的生物医学应用而受到广泛关注。研究了以印楝叶提取物为天然还原剂合成硒纳米粒子（SeNPs）和铁硒双金属纳米粒子（Fe-SeNPs）。通过紫外可见光谱、DLS、FTIR、XRD、SEM、TEM和TGA等手段对合成的纳米颗粒进行了表征，证实了纳米颗粒的成功合成。通过MTT、伤口愈合、细胞凋亡和卵黄囊膜（YSM）检测，评价SeNPs和Fe-SeNPs对MCF-7人乳腺癌细胞的治疗效果。Fe-SeNPs表现出比SeNPs更强的细胞毒性，24 h和48 h的IC50值分别为50 μg/mL和30 μg/mL。Annexin V/PI染色证实，两种纳米颗粒均能显著抑制细胞迁移（SeNPs和Fe-SeNPs在48 h的IC50值分别为56.6%和61.4%），促进细胞凋亡。此外，在YSM实验中观察到对血管生成的剂量依赖性抑制，在较高浓度下完全抑制。这些结果突出了绿色合成的SeNPs和Fe-SeNPs作为乳腺癌治疗的有希望的候选者的潜力，通过诱导细胞毒性，抑制迁移和血管生成，促进细胞凋亡，从而促进了基于纳米颗粒的癌症治疗的发展。

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来源期刊

IEEE Transactions on NanoBioscience 工程技术-纳米科技

CiteScore

7.00

自引率

5.10%

发文量

197

审稿时长

>12 weeks

期刊介绍： The IEEE Transactions on NanoBioscience reports on original, innovative and interdisciplinary work on all aspects of molecular systems, cellular systems, and tissues (including molecular electronics). Topics covered in the journal focus on a broad spectrum of aspects, both on foundations and on applications. Specifically, methods and techniques, experimental aspects, design and implementation, instrumentation and laboratory equipment, clinical aspects, hardware and software data acquisition and analysis and computer based modelling are covered (based on traditional or high performance computing - parallel computers or computer networks).