Development and evaluation of nasal hydrogel with biopolymer loaded gold nanoparticle for antibacterial activity.

IF 3.4 Q2 PHARMACOLOGY & PHARMACY

Therapeutic delivery Pub Date : 2026-02-01 Epub Date: 2026-04-02 DOI:10.1080/20415990.2026.2648379

Thogaikarasi Ravichadran, Selvakumar Rajendran, Sathish Paramasivam Bhama, Punniya Dharshini, Baby Roselin Rajan Durai, Veintramuthu Sankar

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引用次数: 0

Abstract

Aim: This study aimed to develop and evaluate mucoadhesive nasal hydrogels incorporating biopolymer-stabilized gold nanoparticles (GNPs) for antibacterial applications.

Materials and methods: Gold nanoparticles were synthesized by a reduction method and incorporated into hydrogels prepared using carboxymethyl cellulose (CMC; 0.2% and 2%) and pectin (10%). Formulations were characterized for physicochemical interactions, particle size, PDI, zeta potential, viscosity, osmolarity, and FTIR. Mucoadhesive strength was measured using a texture analyzer. Antibacterial activity against Staphylococcus aureus, Pseudomonas aeruginosa, and Klebsiella pneumoniae was evaluated using broth dilution and agar diffusion methods. Cytocompatibility was assessed in RAW 264.7 cells using the MTT assay.

Results: The 2% CMC-GNP hydrogel showed improved stability (-17 to -22 mV) and higher viscosity (700-2000 cP), while pectin-GNP hydrogels exhibited superior mucoadhesion (peak: 1.28 N) and uniform nanoparticle distribution. Both formulations demonstrated antibacterial activity comparable to gentamicin. Cell viability remained high, with pectin-GNP and 2% CMC-GNP showing 95.07% and 90.16%, respectively.

Conclusion: Biopolymer-stabilized GNP hydrogels exhibited enhanced stability, mucoadhesion, antibacterial activity, and biocompatibility, supporting their potential as nasal delivery systems for preventing bacterial colonization.

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生物聚合物载金纳米颗粒鼻用水凝胶的研制及抗菌性能评价。

目的：开发并评价含生物聚合物稳定金纳米颗粒（GNPs）的黏附鼻水凝胶的抗菌应用。材料和方法：采用还原法制备金纳米颗粒，并将其掺入以羧甲基纤维素（0.2%和2%）和果胶（10%）制备的水凝胶中。对配方进行了物理化学相互作用、粒径、PDI、zeta电位、粘度、渗透压和FTIR表征。用织构分析仪测定粘接强度。采用肉汤稀释法和琼脂扩散法评价对金黄色葡萄球菌、铜绿假单胞菌和肺炎克雷伯菌的抑菌活性。使用MTT法评估RAW 264.7细胞的细胞相容性。结果：2% CMC-GNP水凝胶具有较好的稳定性（-17 ~ -22 mV）和较高的粘度（700 ~ 2000 cP），果胶- gnp水凝胶具有较好的黏附性（峰值1.28 N）和均匀的纳米颗粒分布。两种制剂的抗菌活性与庆大霉素相当。细胞存活率较高，果胶gnp和2% CMC-GNP分别为95.07%和90.16%。结论：生物聚合物稳定的GNP水凝胶具有增强的稳定性、黏附性、抗菌活性和生物相容性，支持其作为防止细菌定植的鼻腔给药系统的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Therapeutic delivery PHARMACOLOGY & PHARMACY-

CiteScore

5.50

自引率

0.00%

发文量

期刊介绍： Delivering therapeutics in a way that is right for the patient - safe, painless, reliable, targeted, efficient and cost effective - is the fundamental aim of scientists working in this area. Correspondingly, this evolving field has already yielded a diversity of delivery methods, including injectors, controlled release formulations, drug eluting implants and transdermal patches. Rapid technological advances and the desire to improve the efficacy and safety profile of existing medications by specific targeting to the site of action, combined with the drive to improve patient compliance, continue to fuel rapid research progress. Furthermore, the emergence of cell-based therapeutics and biopharmaceuticals such as proteins, peptides and nucleotides presents scientists with new and exciting challenges for the application of therapeutic delivery science and technology. Successful delivery strategies increasingly rely upon collaboration across a diversity of fields, including biology, chemistry, pharmacology, nanotechnology, physiology, materials science and engineering. Therapeutic Delivery recognizes the importance of this diverse research platform and encourages the publication of articles that reflect the highly interdisciplinary nature of the field. In a highly competitive industry, Therapeutic Delivery provides the busy researcher with a forum for the rapid publication of original research and critical reviews of all the latest relevant and significant developments, and focuses on how the technological, pharmacological, clinical and physiological aspects come together to successfully deliver modern therapeutics to patients. The journal delivers this essential information in concise, at-a-glance article formats that are readily accessible to the full spectrum of therapeutic delivery researchers.