Retroviral Transduction of Human CD4⁺ T Cells with Membrane-Attached IL-10 Generates Type 1-Like Regulatory T Cells.

IF 4 3区医学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Human gene therapy Pub Date : 2026-06-01 Epub Date: 2026-04-02 DOI:10.1177/10430342261437963

Dayana Blokon-Kogan, Shaul Davidson, Maya Levi-Mann, Gideon Gross, Hadas Weinstein-Marom

引用次数: 0

Abstract

Type 1 regulatory T (Tr1) cells are CD4⁺/FoxP3^-/CD49b⁺/LAG-3⁺ regulatory T cells that are induced in the periphery following chronic exposure to antigen in the presence of IL-10. Activated Tr1 cells secrete high quantities of anti-inflammatory cytokines, especially IL-10, and enforce robust bystander immune suppression, rendering them particularly attractive for adoptive cell therapy of autoimmune and autoinflammatory diseases. Here we introduce retroviral transduction of human CD4⁺ T cells with membrane-attached IL-10 (memIL-10) or memIL-10/CAR as a new method for generating large cell populations of high purity, stability, and antigen specificity displaying the main phenotypic and functional characteristics of Tr1 cells.

查看原文本刊更多论文

人CD4+ T细胞与膜附着IL-10的逆转录病毒转导产生1型样调节性T细胞

1型调节性T细胞（Tr1）是CD4+/FoxP3-/CD49b+/LAG-3+调节性T细胞，在IL-10存在下慢性暴露于抗原后在外周诱导。激活的Tr1细胞分泌大量的抗炎细胞因子，特别是IL-10，并执行强大的旁观者免疫抑制，使它们成为自身免疫性和自身炎症性疾病的过继细胞治疗特别有吸引力。本文介绍了利用膜附着IL-10 （memIL-10）或memIL-10/CAR对人CD4+ T细胞进行逆转录病毒转导的新方法，作为产生高纯度、稳定性和抗原特异性的大细胞群的新方法，显示了Tr1细胞的主要表型和功能特征。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Human gene therapy 医学-生物工程与应用微生物

CiteScore

6.50

自引率

4.80%

发文量

131

审稿时长

4-8 weeks

期刊介绍： Human Gene Therapy is the premier, multidisciplinary journal covering all aspects of gene therapy. The Journal publishes in-depth coverage of DNA, RNA, and cell therapies by delivering the latest breakthroughs in research and technologies. Human Gene Therapy provides a central forum for scientific and clinical information, including ethical, legal, regulatory, social, and commercial issues, which enables the advancement and progress of therapeutic procedures leading to improved patient outcomes, and ultimately, to curing diseases.

Retroviral Transduction of Human CD4+ T Cells with Membrane-Attached IL-10 Generates Type 1-Like Regulatory T Cells.

Abstract

Retroviral Transduction of Human CD4⁺ T Cells with Membrane-Attached IL-10 Generates Type 1-Like Regulatory T Cells.