Isolation of Achromobacter sp. SM123 and immobilization of tributyl phosphate-degrading phosphatase enzyme for bioremediation application.

IF 2.1 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology Letters Pub Date : 2026-04-02 DOI:10.1007/s10529-026-03726-4

Soumya Mukundan, Jose Savio Melo, Archana Mishra, Kuber Chandra Bhainsa

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引用次数: 0

Abstract

Tributyl phosphate (TBP) is a solvent and plasticizer commonly used in nuclear fuel reprocessing plants. However, it poses significant environmental challenges due to its stability, persistence, and unique physicochemical properties. To address this, the present study focuses on the biodegradation of TBP by an organism isolated from soil, capable of utilizing TBP as its sole carbon source. Isolated bacteria (Achromobacter sp. SM123) demonstrated efficient TBP degradation, achieving 96% degradation in 29 h following adaptation to butanol. The crude extract contains phosphatase enzyme, responsible for TBP degradation was immobilized onto hydroxyappetite matrix. Various immobilizing parameters were investigated, and storage stability study showed that the immobilized crude extract maintained 69% of its initial activity up to 65 days however the free enzyme retained only 27% activity over 23 days. The phosphatase activity present in crude extract was confirmed by zymogram analysis as a distinct purple band. This work highlights the potential application of isolated bacteria and its immobilized crude extract which contains phosphatase enzyme activity in the bioremediation of TBP-contaminated environment.

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SM123无色杆菌的分离及磷酸三丁酯降解磷酸酶的固定化。

磷酸三丁酯（TBP）是核燃料后处理工厂常用的一种溶剂和增塑剂。然而，由于其稳定性、持久性和独特的物理化学性质，它对环境构成了重大挑战。为了解决这个问题，本研究的重点是通过从土壤中分离出的生物降解TBP，能够利用TBP作为其唯一的碳源。分离的细菌（无色杆菌sp. SM123）显示出高效的TBP降解，在适应丁醇后29小时内降解率达到96%。粗提物中含有降解TBP的磷酸酶，将其固定在羟食欲基质上。研究了不同的固定化参数，并对固定化粗提物的贮存稳定性进行了研究，结果表明，固定化粗提物在65 d内保持了69%的初始活性，而在23 d内游离酶仅保持了27%的活性。酶谱分析证实了粗提物中存在的磷酸酶活性呈明显的紫色带。本研究强调了分离菌及其含磷酸酶活性的固定化粗提物在tbp污染环境的生物修复中的潜在应用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biotechnology Letters 工程技术-生物工程与应用微生物

CiteScore

5.90

自引率

3.70%

发文量

108

审稿时长

1.2 months

期刊介绍： Biotechnology Letters is the world’s leading rapid-publication primary journal dedicated to biotechnology as a whole – that is to topics relating to actual or potential applications of biological reactions affected by microbial, plant or animal cells and biocatalysts derived from them. All relevant aspects of molecular biology, genetics and cell biochemistry, of process and reactor design, of pre- and post-treatment steps, and of manufacturing or service operations are therefore included. Contributions from industrial and academic laboratories are equally welcome. We also welcome contributions covering biotechnological aspects of regenerative medicine and biomaterials and also cancer biotechnology. Criteria for the acceptance of papers relate to our aim of publishing useful and informative results that will be of value to other workers in related fields. The emphasis is very much on novelty and immediacy in order to justify rapid publication of authors’ results. It should be noted, however, that we do not normally publish papers (but this is not absolute) that deal with unidentified consortia of microorganisms (e.g. as in activated sludge) as these results may not be easily reproducible in other laboratories. Papers describing the isolation and identification of microorganisms are not regarded as appropriate but such information can be appended as supporting information to a paper. Papers dealing with simple process development are usually considered to lack sufficient novelty or interest to warrant publication.