Diversity-triggered 2-naphthoic acid exudation recruits keystone microbial taxa to promote soybean drought tolerance

IF 18.7 1区医学 Q1 MICROBIOLOGY

Cell host & microbe Pub Date : 2026-03-30 DOI:10.1016/j.chom.2026.03.002

Shi Chen, Yang Wang, Beibei Chen, Xinyi Hou, Siyu Liu, Sihan He, Jiejun Qi, Ziheng Peng, Haibo Pan, Chunling Liang, Gehong Wei, Shuo Jiao

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引用次数: 0

Abstract

Rhizosphere microbiomes are essential for plant growth and stress tolerance, yet how microbial diversity shapes drought resilience in soybean remains unclear. Here, we demonstrate that high rhizosphere microbial diversity, generated via dilution-to-extinction manipulation of soil microbiome diversity, improves soybean performance under drought. Integrated metabolomic and transcriptomic analyses identify 2-naphthoic acid as a diversity-induced root exudate that accumulates exclusively under drought. This metabolite selectively recruits Sinorhizobium CS204 via chemotaxis and ATP-binding cassette (ABC) transporter-mediated uptake, as confirmed by in vitro substrate utilization assays and targeted mutant construction. Molecular docking and microscale thermophoresis reveal direct interactions between this metabolite and nitrogen-cycling proteins, enhancing denitrification and nitrogen fixation of S.CS204. Co-application of 2-naphthoic acid and S.CS204 significantly improves plant nutrient acquisition and photosynthesis under drought. Collectively, our study underscores the pivotal role of rhizosphere microbial diversity in triggering the exudation of root metabolites to recruit keystone taxa, establishing microbe-plant synergies that bolster drought tolerance.

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多样性引发的2-萘酸分泌为大豆抗旱性提升提供了关键的微生物类群

根际微生物群对植物生长和抗逆性至关重要，但微生物多样性如何影响大豆的抗旱性尚不清楚。本研究表明，通过对土壤微生物群落多样性进行稀释到灭绝的处理，提高了大豆在干旱条件下的生产性能。综合代谢组学和转录组学分析表明，2-萘酸是一种多样性诱导的根系分泌物，只在干旱条件下积累。体外底物利用试验和靶向突变体构建证实，该代谢物通过趋化性和atp结合盒（ABC）转运体介导的摄取选择性地招募Sinorhizobium CS204。分子对接和微尺度热电泳揭示了该代谢物与氮循环蛋白之间的直接相互作用，增强了S.CS204的反硝化和固氮作用。2-萘酸与S.CS204配施显著提高了干旱条件下植物的养分获取和光合作用。总的来说，我们的研究强调了根际微生物多样性在触发根代谢物分泌以招募关键分类群方面的关键作用，建立了增强耐旱性的微生物-植物协同作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cell host & microbe 生物-微生物学

CiteScore

45.10

自引率

1.70%

发文量

201

审稿时长

4-8 weeks

期刊介绍： Cell Host & Microbe is a scientific journal that was launched in March 2007. The journal aims to provide a platform for scientists to exchange ideas and concepts related to the study of microbes and their interaction with host organisms at a molecular, cellular, and immune level. It publishes novel findings on a wide range of microorganisms including bacteria, fungi, parasites, and viruses. The journal focuses on the interface between the microbe and its host, whether the host is a vertebrate, invertebrate, or plant, and whether the microbe is pathogenic, non-pathogenic, or commensal. The integrated study of microbes and their interactions with each other, their host, and the cellular environment they inhabit is a unifying theme of the journal. The published work in Cell Host & Microbe is expected to be of exceptional significance within its field and also of interest to researchers in other areas. In addition to primary research articles, the journal features expert analysis, commentary, and reviews on current topics of interest in the field.