Identification, Pathogenicity, and Fungicide Sensitivity of Sclerotinia sclerotiorum Isolates Causing White Mould on Cucumber in Sinaloa, Mexico

IF 1.1 4区农林科学 Q3 PLANT SCIENCES

Journal of Phytopathology Pub Date : 2026-03-24 DOI:10.1111/jph.70287

María B. Contreras-Soto, Guillermo Márquez-Licona, Alma R. Solano-Báez, José A. Carrillo-Fasio, Isabel Cruz-Lachica, Isidro Márquez-Zequera, Raymundo S. García-Estrada, Juan M. Tovar-Pedraza

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Abstract

In January 2025, severe white mould symptoms were observed on cucumber fruits (Cucumis sativus L.) from plants grown under shade netting in Navolato, Sinaloa, Mexico. Morphological characterisation enabled the identification of Sclerotinia sclerotiorum, and its identity was confirmed through phylogenetic analysis using ITS region sequences. Isolates of S. sclerotiorum were inoculated onto cucumber fruits (European and pickling types), producing typical symptoms of fruit rot and white mould. In the in vitro sensitivity assay, five S. sclerotiorum isolates were evaluated and exhibited EC₅₀ value ranges of 0.0098–0.0204, 0.0068–0.0570, 0.0006–0.0031, and 0.0968–3.1648 μg mL⁻¹ for the fungicides pydiflumetofen, pyraclostrobin, fluazinam, and thiophanate-methyl, respectively. This study provides relevant information for the diagnosis and management of white mould in cucumber crops.

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墨西哥锡那罗亚州黄瓜白霉菌核菌分离株的鉴定、致病性及杀菌剂敏感性

2025年1月，在墨西哥锡那罗亚州纳沃拉托遮阳网种植的黄瓜果实（Cucumis sativus L.）出现了严重的白霉病。形态学鉴定鉴定了该菌核菌，并利用its区序列进行了系统发育分析。将菌核菌分离株接种于黄瓜果实（欧式和酸洗型）上，产生典型的果实腐烂和白霉病症状。在体外敏感性试验中，5株菌核菌分离株对杀菌剂吡氟醚、吡氯菌酯、氟嗪南和甲基硫代菌酯的EC50值分别为0.0098 ~ 0.0204、0.0068 ~ 0.0570、0.0006 ~ 0.0031和0.0968 ~ 3.1648 μ mL−1。本研究为黄瓜白霉病的诊断和管理提供了相关信息。

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来源期刊

Journal of Phytopathology 生物-植物科学

CiteScore

2.90

自引率

0.00%

发文量

审稿时长

4-8 weeks

期刊介绍： Journal of Phytopathology publishes original and review articles on all scientific aspects of applied phytopathology in agricultural and horticultural crops. Preference is given to contributions improving our understanding of the biotic and abiotic determinants of plant diseases, including epidemics and damage potential, as a basis for innovative disease management, modelling and forecasting. This includes practical aspects and the development of methods for disease diagnosis as well as infection bioassays. Studies at the population, organism, physiological, biochemical and molecular genetic level are welcome. The journal scope comprises the pathology and epidemiology of plant diseases caused by microbial pathogens, viruses and nematodes. Accepted papers should advance our conceptual knowledge of plant diseases, rather than presenting descriptive or screening data unrelated to phytopathological mechanisms or functions. Results from unrepeated experimental conditions or data with no or inappropriate statistical processing will not be considered. Authors are encouraged to look at past issues to ensure adherence to the standards of the journal.