Hybrid Microtubule-Solid-State Nanopores for Single-Molecule Analysis.

IF 2.5 3区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

ELECTROPHORESIS Pub Date : 2026-03-29 DOI:10.1002/elps.70091

Matthew O'Donohue, Chaoming Gu, Byungsoo Kim, KeunMin Ken Lee, Sangyoup Lee, Chi Won Ahn, Myung Chul Choi, Min Jun Kim

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引用次数: 0

Abstract

We demonstrate a hybrid microtubule-solid-state nanopore (MT-SSN) platform that enables label-free single-molecule analysis. Under continuous voltage bias, individual taxol-stabilized MTs are electrostatically anchored into an SSN to form a stable conduit for ionic current. We measured ionic current through MT-SSNs under two distinct configurations, one in which the SSN constricts ionic flow, and the other where the MT itself serves as the primary conduction channel. The geometrical asymmetry of the hybrid MT-SSN leads to a pronounced current-voltage asymmetry, that is, current rectification. Compared to bare SSNs, the hybrid MT-SSN slows double-stranded DNA translocation by up to ∼3.5× and enhances event-level signal contrast by increasing the relative current blockade, despite an increase in baseline low-frequency noise. By repurposing the hollow, charged nanotubule of MTs to establish a novel framework for probing nanoscale ionic transport at the single-molecule level, this study provides insight into the broader use of cytoskeletal proteins for bioelectronics sensing.

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用于单分子分析的混合微管-固态纳米孔。

我们展示了一种混合微管-固态纳米孔（MT-SSN）平台，可以实现无标签的单分子分析。在持续的电压偏置下，单个紫杉醇稳定的mt被静电锚定在SSN中，形成一个稳定的离子电流管道。我们在两种不同的配置下通过MT-SSN测量离子电流，其中SSN限制离子流动，而另一种MT本身作为主要传导通道。混合MT-SSN的几何不对称导致明显的电流-电压不对称，即电流整流。与裸ssn相比，混合MT-SSN减缓双链DNA易位高达3.5倍，并通过增加相对电流阻断来增强事件级信号对比度，尽管基线低频噪声增加。通过重新利用MTs的空心、带电纳米管来建立一个新的框架，在单分子水平上探测纳米级离子传输，本研究为细胞骨架蛋白在生物电子传感中的更广泛应用提供了见解。

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来源期刊

ELECTROPHORESIS 生物-分析化学

CiteScore

6.30

自引率

13.80%

发文量

244

审稿时长

1.9 months

期刊介绍： ELECTROPHORESIS is an international journal that publishes original manuscripts on all aspects of electrophoresis, and liquid phase separations (e.g., HPLC, micro- and nano-LC, UHPLC, micro- and nano-fluidics, liquid-phase micro-extractions, etc.). Topics include new or improved analytical and preparative methods, sample preparation, development of theory, and innovative applications of electrophoretic and liquid phase separations methods in the study of nucleic acids, proteins, carbohydrates natural products, pharmaceuticals, food analysis, environmental species and other compounds of importance to the life sciences. Papers in the areas of microfluidics and proteomics, which are not limited to electrophoresis-based methods, will also be accepted for publication. Contributions focused on hyphenated and omics techniques are also of interest. Proteomics is within the scope, if related to its fundamentals and new technical approaches. Proteomics applications are only considered in particular cases. Papers describing the application of standard electrophoretic methods will not be considered. Papers on nanoanalysis intended for publication in ELECTROPHORESIS should focus on one or more of the following topics: • Nanoscale electrokinetics and phenomena related to electric double layer and/or confinement in nano-sized geometry • Single cell and subcellular analysis • Nanosensors and ultrasensitive detection aspects (e.g., involving quantum dots, "nanoelectrodes" or nanospray MS) • Nanoscale/nanopore DNA sequencing (next generation sequencing) • Micro- and nanoscale sample preparation • Nanoparticles and cells analyses by dielectrophoresis • Separation-based analysis using nanoparticles, nanotubes and nanowires.