3D Hydrogel Cell Cultures and Their Biomedical Applications

IF 4.8 Q2 ENGINEERING, BIOMEDICAL

Advanced Nanobiomed Research Pub Date : 2026-03-03 Epub Date: 2025-12-08 DOI:10.1002/anbr.202500133

Tri Lan Thai, Arushi Manchanda, Sarah L. Higginbottom, Estrella Sanz Rodriguez, Richard Wilson, Eva Tomaskovic-Crook, Jeremy M. Crook, Brett Paull

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Abstract

In recent years, research into 3D cell cultures has grown remarkably, supporting significant advances in biomedical applications and drug development. Numerous studies have demonstrated their importance for controlling the cellular environment to better emulate natural tissue conditions and provide new insights into cell–cell and cell–matrix interactions for cell growth, differentiation, and migration. This review explores how 3D cell cultures outperform traditional planar/2D culture systems, detailing their key characteristics along with the strengths and limitations of different hydrogel biomaterials and platforms used for their formation, housing, and studying such cell-in-hydrogel cultures, including microfluidic and 3D printed arrangements. Finally, the review showcases recent cutting-edge applications of 3D hydrogel models and their essential roles in biomedical research and translation, including tissue engineering, drug discovery, cancer research, and neuroscience.

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三维水凝胶细胞培养及其生物医学应用

近年来，对3D细胞培养的研究取得了显著进展，支持了生物医学应用和药物开发的重大进展。许多研究已经证明了它们对于控制细胞环境以更好地模拟自然组织条件的重要性，并为细胞生长，分化和迁移的细胞-细胞和细胞-基质相互作用提供了新的见解。这篇综述探讨了3D细胞培养如何优于传统的平面/2D培养系统，详细介绍了它们的关键特征，以及用于形成、容纳和研究这种水凝胶细胞培养的不同水凝胶生物材料和平台的优势和局限性，包括微流体和3D打印安排。最后，综述展示了3D水凝胶模型的最新前沿应用及其在生物医学研究和转化中的重要作用，包括组织工程、药物发现、癌症研究和神经科学。

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来源期刊

Advanced Nanobiomed Research nanomedicine, bioengineering and biomaterials-

CiteScore

5.00

自引率

5.90%

发文量

审稿时长

21 weeks

期刊介绍： Advanced NanoBiomed Research will provide an Open Access home for cutting-edge nanomedicine, bioengineering and biomaterials research aimed at improving human health. The journal will capture a broad spectrum of research from increasingly multi- and interdisciplinary fields of the traditional areas of biomedicine, bioengineering and health-related materials science as well as precision and personalized medicine, drug delivery, and artificial intelligence-driven health science. The scope of Advanced NanoBiomed Research will cover the following key subject areas: ▪ Nanomedicine and nanotechnology, with applications in drug and gene delivery, diagnostics, theranostics, photothermal and photodynamic therapy and multimodal imaging. ▪ Biomaterials, including hydrogels, 2D materials, biopolymers, composites, biodegradable materials, biohybrids and biomimetics (such as artificial cells, exosomes and extracellular vesicles), as well as all organic and inorganic materials for biomedical applications. ▪ Biointerfaces, such as anti-microbial surfaces and coatings, as well as interfaces for cellular engineering, immunoengineering and 3D cell culture. ▪ Biofabrication including (bio)inks and technologies, towards generation of functional tissues and organs. ▪ Tissue engineering and regenerative medicine, including scaffolds and scaffold-free approaches, for bone, ligament, muscle, skin, neural, cardiac tissue engineering and tissue vascularization. ▪ Devices for healthcare applications, disease modelling and treatment, such as diagnostics, lab-on-a-chip, organs-on-a-chip, bioMEMS, bioelectronics, wearables, actuators, soft robotics, and intelligent drug delivery systems. with a strong focus on applications of these fields, from bench-to-bedside, for treatment of all diseases and disorders, such as infectious, autoimmune, cardiovascular and metabolic diseases, neurological disorders and cancer; including pharmacology and toxicology studies.