The effect of the secretory phospholipases A2 from Gloydius blomhoffii and Gloydius halys venoms on hemostasis components in vitro.

Q3 Biochemistry, Genetics and Molecular Biology

Biomeditsinskaya khimiya Pub Date : 2026-02-01 DOI:10.18097/PBMCR1639

M A Solovyeva, L V Galebskaya, M A Galkin, G M Berdichevskiy, E V Semenova, I M Mikhel, E A Rogozhin, L V Vasina

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引用次数: 0

Abstract

The aim of this study was to investigate the effects of Gloydius blomhoffii and Gloydius halys venoms and their fractions on platelet and plasma hemostasis, and to evaluate the ability of the sPLA2 inhibitor Varespladib to neutralize the coagulopathic activity mediated by secretory phospholipase A2 (sPLA2) present in these venoms. The proaggregatory effect of whole G. halys and G. blomhoffii venoms on platelet hemostasis was associated with multidirectional effects of their multiple protein components, while sPLA2-containing fractions exhibited an antiaggregatory effect. Whole G. halys and G. blomhoffii venoms and Varespladib added to them did not affect the extrinsic pathway of hemocoagulation, but inhibited the intrinsic pathway of human blood coagulation. The paradoxical effect of further enhancing the anticoagulant activity of G. halys and G. blomhoffii venoms with the sPLA2 inhibitor Varespladib could be determined by a stronger sPLA2 interaction with blood coagulation factor Xa after formation of the enzyme-inhibitor complex.

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黄颡鱼和黄颡鱼毒液分泌磷脂酶A2对体外止血成分的影响。

本研究的目的是研究黄颡鱼和黄颡鱼毒液及其组分对血小板和血浆止血的影响，并评估sPLA2抑制剂Varespladib对这些毒液中由分泌磷脂酶A2 （sPLA2）介导的凝血活性的中和能力。黄颡鱼和黄颡鱼全毒液对血小板止血的促聚集作用与其多种蛋白组分的多向作用有关，而含spla2的部分具有抗聚集作用。在其中添加全黄颡鱼和黄颡鱼毒液及缬沙替布对人血液凝固的外在途径没有影响，但对人血液凝固的内在途径有抑制作用。sPLA2抑制剂Varespladib进一步增强白蛉和布氏白蛉毒液抗凝血活性的矛盾效应可以通过sPLA2与凝血因子Xa形成酶抑制剂复合物后更强的相互作用来确定。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biomeditsinskaya khimiya Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)

CiteScore

1.30

自引率

0.00%

发文量

期刊介绍： The aim of the Russian-language journal "Biomeditsinskaya Khimiya" (Biomedical Chemistry) is to introduce the latest results obtained by scientists from Russia and other Republics of the Former Soviet Union. The Journal will cover all major areas of Biomedical chemistry, including neurochemistry, clinical chemistry, molecular biology of pathological processes, gene therapy, development of new drugs and their biochemical pharmacology, introduction and advertisement of new (biochemical) methods into experimental and clinical medicine etc. The Journal also publish review articles. All issues of journal usually contain invited reviews. Papers written in Russian contain abstract (in English).