Amplification-free reading of double-stranded DNA.

IF 16.3 2区生物学 Q1 GENETICS & HEREDITY

Trends in Genetics Pub Date : 2026-05-01 Epub Date: 2026-03-19 DOI:10.1016/j.tig.2026.02.009

Meidie Pan, Luoran Shang

引用次数: 0

Abstract

Detecting double-stranded DNA sequences typically requires enzymes to unwind and amplify the target. A new study by Yan et al. challenges this view by combining gamma peptide nucleic acid with multicomponent deoxyribozyme in droplets, achieving single-molecule sensitivity without protein enzymes or amplification, enabling concise, economical, and highly sensitive genetic diagnosis.

查看原文本刊更多论文

双链DNA的无扩增读取。

检测双链DNA序列通常需要酶解开并扩增目标。Yan等人的一项新研究挑战了这一观点，他们将γ肽核酸与多组分脱氧核酶结合在液滴中，无需蛋白酶或扩增即可实现单分子灵敏度，从而实现简洁、经济、高灵敏度的遗传诊断。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Trends in Genetics 生物-遗传学

CiteScore

20.90

自引率

0.90%

发文量

160

审稿时长

6-12 weeks

期刊介绍： Launched in 1985, Trends in Genetics swiftly established itself as a "must-read" for geneticists, offering concise, accessible articles covering a spectrum of topics from developmental biology to evolution. This reputation endures, making TiG a cherished resource in the genetic research community. While evolving with the field, the journal now embraces new areas like genomics, epigenetics, and computational genetics, alongside its continued coverage of traditional subjects such as transcriptional regulation, population genetics, and chromosome biology. Despite expanding its scope, the core objective of TiG remains steadfast: to furnish researchers and students with high-quality, innovative reviews, commentaries, and discussions, fostering an appreciation for advances in genetic research. Each issue of TiG presents lively and up-to-date Reviews and Opinions, alongside shorter articles like Science & Society and Spotlight pieces. Invited from leading researchers, Reviews objectively chronicle recent developments, Opinions provide a forum for debate and hypothesis, and shorter articles explore the intersection of genetics with science and policy, as well as emerging ideas in the field. All articles undergo rigorous peer-review.