Overexpression of wheat Wcor80 confers tolerance to low temperature in transgenic plant.

IF 1.9 3区 生物学 Q2 BIOCHEMICAL RESEARCH METHODS
Luyao Wang, Jiaang Cao, Xuesong Wang, Junbao Zhang, Yushu Chen, Sen Yang, Qingyi Shao, Mengdi Yu, Zhongmin Jin, Lijie Liu
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引用次数: 0

Abstract

Low temperature is a common abiotic stress, which can seriously restrict the normal growth and development of crops. In this study, wheat cold-regulated gene 80 (Wcor80) was isolated from "Dongnongdongmai 1" (Dn1) material and characterized. The expression of Wcor80 was induced by brassinolide (BR) and low temperature (LT) treatments, and it was preferentially expressed in the leaves under abscisic acid (ABA) treatment and in the tillering nodes under BR treatment. Overexpression of Wcor80 in transgenic Arabidopsis significantly increased tolerance to LT treatment. Overall, our findings suggest that Wcor80 functions as a positive regulator of resistance to LT stress, providing a theoretical basis for the molecular breeding and cultivation regulation of winter wheat.

小麦Wcor80基因的过表达使转基因植株具有耐低温性。
低温是一种常见的非生物胁迫,严重制约了作物的正常生长发育。从“东农东麦1号”(Dn1)材料中分离到小麦冷调控基因80 (Wcor80),并对其进行了鉴定。Wcor80在油菜素内酯(BR)和低温(LT)处理下均有诱导表达,且在脱落酸(ABA)处理下优先在叶片中表达,在BR处理下优先在分蘖节中表达。转基因拟南芥中过表达Wcor80显著增加了对LT处理的耐受性。综上所述,我们的研究结果表明Wcor80是小麦抗LT胁迫的正向调控因子,为冬小麦分子育种和栽培调控提供了理论依据。
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来源期刊
Transgenic Research
Transgenic Research 生物-生化研究方法
CiteScore
5.40
自引率
0.00%
发文量
38
审稿时长
4-8 weeks
期刊介绍: Transgenic Research focusses on transgenic and genome edited higher organisms. Manuscripts emphasizing biotechnological applications are strongly encouraged. Intellectual property, ethical issues, societal impact and regulatory aspects also fall within the scope of the journal. Transgenic Research aims to bridge the gap between fundamental and applied science in molecular biology and biotechnology for the plant and animal academic and associated industry communities. Transgenic Research publishes -Original Papers -Reviews: Should critically summarize the current state-of-the-art of the subject in a dispassionate way. Authors are requested to contact a Board Member before submission. Reviews should not be descriptive; rather they should present the most up-to-date information on the subject in a dispassionate and critical way. Perspective Reviews which can address new or controversial aspects are encouraged. -Brief Communications: Should report significant developments in methodology and experimental transgenic higher organisms
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