Oligosaccharide oxidase for the enzymatic synthesis of glucosaminic acids.

IF 2.1 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology Letters Pub Date : 2026-03-17 DOI:10.1007/s10529-026-03721-9

Olanrewaju Raji, Thu V Vuong, Nadia Davoudvandi, Emma R Master

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引用次数: 0

Abstract

D-Glucosaminic acid is a valuable amino acid useful in food and medical applications. It is a highly sought after enantiopure molecule important for the synthesis of drugs and glycopeptides. Current enzymatic synthesis pathways to D-glucosaminic acid carry disadvantages such as low product yield and long reaction times. Herein, the Auxiliary Activity 7 chito-oligosaccharide oxidase from Lentinus brumalis, LbChi7A, was shown as a potent biocatalyst capable of efficiently converting D-glucosamine (GlcN) and N-acetyl-D-glucosamine (GlcNAc) to their respective C₁-acids. The substrate specificity of LbChi7A towards GlcN and GlcNAc enabled the conversion of at least 90% GlcN to D-glucosaminic acid and 100% GlcNAc to N-acetyl-D-glucosaminic acid within 60 min. LbChi7A inhibition by the hydrogen peroxide co-product was not detected, even at 860 mM. This single enzymatic conversion offers a clean and efficient process to produce industrially relevant glucosaminic acids, including D-glucosaminic acid and N-acetyl-D-glucosaminic acid.

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低聚糖氧化酶，用于酶促合成氨基葡萄糖酸。

d -氨基葡萄糖是一种有价值的氨基酸，在食品和医疗应用中都很有用。它是一种备受追捧的对映不纯分子，对药物和糖肽的合成很重要。目前酶法合成d -氨基葡萄糖的方法存在产率低、反应时间长等缺点。本研究表明，从香菇中提取的辅助活性7壳寡糖氧化酶LbChi7A是一种有效的生物催化剂，能够有效地将d -氨基葡萄糖（GlcN）和n -乙酰- d -氨基葡萄糖（GlcNAc）转化为各自的c1 -酸。LbChi7A对GlcN和GlcNAc的底物特异性使得至少90%的GlcN在60分钟内转化为d -氨基葡萄糖酸，100%的GlcNAc转化为n -乙酰- d -氨基葡萄糖酸。即使在860 mM的温度下，也没有检测到过氧化氢副产物对LbChi7A的抑制作用。这种单酶转化提供了一种清洁高效的工艺来生产工业相关的氨基葡萄糖酸，包括d -氨基葡萄糖酸和n -乙酰- d -氨基葡萄糖酸。

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来源期刊

Biotechnology Letters 工程技术-生物工程与应用微生物

CiteScore

5.90

自引率

3.70%

发文量

108

审稿时长

1.2 months

期刊介绍： Biotechnology Letters is the world’s leading rapid-publication primary journal dedicated to biotechnology as a whole – that is to topics relating to actual or potential applications of biological reactions affected by microbial, plant or animal cells and biocatalysts derived from them. All relevant aspects of molecular biology, genetics and cell biochemistry, of process and reactor design, of pre- and post-treatment steps, and of manufacturing or service operations are therefore included. Contributions from industrial and academic laboratories are equally welcome. We also welcome contributions covering biotechnological aspects of regenerative medicine and biomaterials and also cancer biotechnology. Criteria for the acceptance of papers relate to our aim of publishing useful and informative results that will be of value to other workers in related fields. The emphasis is very much on novelty and immediacy in order to justify rapid publication of authors’ results. It should be noted, however, that we do not normally publish papers (but this is not absolute) that deal with unidentified consortia of microorganisms (e.g. as in activated sludge) as these results may not be easily reproducible in other laboratories. Papers describing the isolation and identification of microorganisms are not regarded as appropriate but such information can be appended as supporting information to a paper. Papers dealing with simple process development are usually considered to lack sufficient novelty or interest to warrant publication.