Research progress on direct PCR and its applications

IF 2 4区化学 Q3 CHEMISTRY, ANALYTICAL

Analytical Sciences Pub Date : 2026-03-17 DOI:10.1007/s44211-026-00893-2

Ting Xu, Siyu Yang, Yingqiu Pan, Xingjuan Hu, Feng Guan, Huili Xia

引用次数: 0

Abstract

Direct Amplification Technology (Direct PCR) is derived from conventional PCR and eliminates the cumbersome DNA extraction process, thereby enhancing detection efficiency and accuracy. Combined with evolving PCR-derived techniques, it has found extensive applications across multiple fields. This article provides an overview of the development process, fundamental principles, basic procedures, and derivative technologies of direct PCR. It further details its applications in testing various samples including blood, microbial specimens, and cellular/tissue samples, offering foundational knowledge to support the popularization and further advancement of this technology.

AbstractSection Graphical Abstract

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直接PCR及其应用研究进展。

直接扩增技术（Direct PCR）是在传统PCR的基础上发展起来的，消除了繁琐的DNA提取过程，提高了检测效率和准确性。结合不断发展的pcr衍生技术，它在多个领域得到了广泛的应用。本文综述了直接PCR的发展过程、基本原理、基本步骤和衍生技术。它进一步详细介绍了其在测试各种样品中的应用，包括血液、微生物标本和细胞/组织样品，为支持该技术的普及和进一步发展提供了基础知识。此图像的替代文本可能是使用AI生成的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Analytical Sciences 化学-分析化学

CiteScore

2.90

自引率

18.80%

发文量

232

审稿时长

1 months

期刊介绍： Analytical Sciences is an international journal published monthly by The Japan Society for Analytical Chemistry. The journal publishes papers on all aspects of the theory and practice of analytical sciences, including fundamental and applied, inorganic and organic, wet chemical and instrumental methods. This publication is supported in part by the Grant-in-Aid for Publication of Scientific Research Result of the Japanese Ministry of Education, Culture, Sports, Science and Technology.