Enzymatic assay in a lab-on-a-disk format to measure free piperacillin concentration in serum

IF 3.3 4区医学 Q3 ENGINEERING, BIOMEDICAL

Biomedical Microdevices Pub Date : 2026-03-17 DOI:10.1007/s10544-026-00807-z

Olivier Verlaine, Ana Amoroso, Maxence Remy, Stéphanie Wautier, Raphaël Robiette, Bernard Joris, Tristan Gilet

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Abstract

This work describes a method based on an enzymatic assay to measure the free concentration of piperacillin (a \(\varvec{\beta }\)-lactam antibiotics) in blood serum. The assay is based on the release of fluorescent umbelliferone, upon hydrolysis by BlaP99 \(\varvec{\beta }\)-lactamase of a substrate in competition with piperacillin. The assay is implemented in a lab-on-a-disk (LoaD) setup, in which samples and reagents are manipulated with robust centrifugal microfluidic techniques. To encounter the needs and constraints of Therapeutic Drug Monitoring (TDM) at bedside, the system is entirely automated and miniaturized. Within a few minutes and from a few microliters of serum, it provides piperacillin concentration measurements in a clinically meaningful range. This paper describes the design and characterization of the chemical kinetics and the microfluidic strategy underlying this assay. The data are compared to measurements made in well-plates with a conventional method.

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酶测定法在实验室磁盘格式测量游离哌拉西林浓度在血清中。

本工作描述了一种基于酶法测定血清中哌拉西林（a[公式：见文本]-内酰胺类抗生素）游离浓度的方法。该分析是基于荧光伞形酮的释放，在与哌拉西林竞争的底物的BlaP99[公式：见文本]-内酰胺酶水解。该分析在磁盘上的实验室（LoaD）设置中实现，其中样品和试剂用稳健的离心微流体技术进行操作。为了满足床边治疗药物监测（TDM）的需求和限制，该系统是完全自动化和小型化的。在几分钟内，从几微升血清，它提供哌拉西林浓度测量在临床有意义的范围。本文描述了设计和表征的化学动力学和微流控策略的基础上，这一分析。这些数据与用常规方法在井板上测量的数据进行了比较。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biomedical Microdevices 工程技术-工程：生物医学

CiteScore

6.90

自引率

3.60%

发文量

审稿时长

6 months

期刊介绍： Biomedical Microdevices: BioMEMS and Biomedical Nanotechnology is an interdisciplinary periodical devoted to all aspects of research in the medical diagnostic and therapeutic applications of Micro-Electro-Mechanical Systems (BioMEMS) and nanotechnology for medicine and biology. General subjects of interest include the design, characterization, testing, modeling and clinical validation of microfabricated systems, and their integration on-chip and in larger functional units. The specific interests of the Journal include systems for neural stimulation and recording, bioseparation technologies such as nanofilters and electrophoretic equipment, miniaturized analytic and DNA identification systems, biosensors, and micro/nanotechnologies for cell and tissue research, tissue engineering, cell transplantation, and the controlled release of drugs and biological molecules. Contributions reporting on fundamental and applied investigations of the material science, biochemistry, and physics of biomedical microdevices and nanotechnology are encouraged. A non-exhaustive list of fields of interest includes: nanoparticle synthesis, characterization, and validation of therapeutic or imaging efficacy in animal models; biocompatibility; biochemical modification of microfabricated devices, with reference to non-specific protein adsorption, and the active immobilization and patterning of proteins on micro/nanofabricated surfaces; the dynamics of fluids in micro-and-nano-fabricated channels; the electromechanical and structural response of micro/nanofabricated systems; the interactions of microdevices with cells and tissues, including biocompatibility and biodegradation studies; variations in the characteristics of the systems as a function of the micro/nanofabrication parameters.