Placental Oxidative Stress and eNOS Alterations in Intrahepatic Cholestasis of Pregnancy Following ICSI.

IF 0.8 4区医学 Q4 PATHOLOGY

Fetal and Pediatric Pathology Pub Date : 2026-03-01 Epub Date: 2026-03-16 DOI:10.1080/15513815.2026.2640497

Sibel Bulgurcuoglu Kuran, Meryem Hocaoglu, Ayse Altun, Selin Demirer, Ibrahim Sogut, Sezen Gizem Aydogan, Ilayda Loçlar Karaalp, Elvan Ozturk, Esra Kaynak, Abdulkadir Turgut, Evrim Komurcu-Bayrak, Canan Hurdag

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引用次数: 0

Abstract

Background: Pregnancies following in vitro fertilization (IVF) are associated with higher rates of intrahepatic cholestasis of pregnancy (ICP) compared to spontaneous conceptions. This study aimed to investigate oxidative stress biomarkers, endothelial nitric oxide synthase (eNOS) expression, and placental histopathology in ICP women undergoing intracytoplasmic sperm injection (ICSI). Methods: Placental samples from four groups-healthy spontaneous conception (non-ICP-SC, n = 7), ICP spontaneous conception (ICP-SC, n = 7), healthy ICSI (non-ICP-ICSI, n = 7), and ICP-ICSI (n = 7)-were analyzed. eNOS immunostaining, total antioxidant status (TAS), total oxidant status (TOS), oxidative stress index (OSI), and syncytial knots were evaluated. Results: The ICP ICSI group had the highest TAS and lowest eNOS levels (p < 0.05). Non-ICP-ICSI showed higher TOS and syncytial knots compared to non-ICP-SC (p = 0.026, p = 0.01). eNOS expression was lower in ICP-ICSI versus ICP-SC (p = 0.021). Conclusion: These findings suggest that ICP-ICSI placentas experience increased oxidative stress and decreased eNOS expression. ICSI may affect placental oxidative balance regardless of ICP status.

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ICSI后妊娠肝内胆汁淤积的胎盘氧化应激和eNOS改变。

背景：与自然受孕相比，体外受精（IVF）妊娠与妊娠肝内胆汁淤积（ICP）的发生率较高相关。本研究旨在探讨接受胞浆内单精子注射（ICSI）的ICP妇女的氧化应激生物标志物、内皮一氧化氮合酶（eNOS）表达和胎盘组织病理学。方法：对健康自然受孕（非ICP- sc， n = 7）、ICP自然受孕（ICP- sc, n = 7）、健康ICSI（非ICP-ICSI， n = 7）、ICP-ICSI （n = 7）四组胎盘样本进行分析。评估eNOS免疫染色、总抗氧化状态（TAS）、总氧化状态（TOS）、氧化应激指数（OSI）和合胞结。结果：ICP ICSI组TAS最高，eNOS最低（p < 0.05）。与非icp - sc相比，非icp - icsi组TOS和合胞结较高（p = 0.026, p = 0.01）。与ICP-SC相比，ICP-ICSI中eNOS表达较低（p = 0.021）。结论：这些结果提示ICP-ICSI胎盘氧化应激增加，eNOS表达降低。无论ICP状态如何，ICSI都可能影响胎盘氧化平衡。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Fetal and Pediatric Pathology 医学-病理学

CiteScore

3.00

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Fetal and Pediatric Pathology is an established bimonthly international journal that publishes data on diseases of the developing embryo, newborns, children, and adolescents. The journal publishes original and review articles and reportable case reports. The expanded scope of the journal encompasses molecular basis of genetic disorders; molecular basis of diseases that lead to implantation failures; molecular basis of abnormal placentation; placentology and molecular basis of habitual abortion; intrauterine development and molecular basis of embryonic death; pathogenisis and etiologic factors involved in sudden infant death syndrome; the underlying molecular basis, and pathogenesis of diseases that lead to morbidity and mortality in newborns; prenatal, perinatal, and pediatric diseases and molecular basis of diseases of childhood including solid tumors and tumors of the hematopoietic system; and experimental and molecular pathology.