Mutual feedback regulation between Poly(A)-specific ribonuclease (PARN) and cognate microRNAs.

IF 3.1 2区生物学 Q1 BIOLOGY

Life Science Alliance Pub Date : 2026-03-13 Print Date: 2026-05-01 DOI:10.26508/lsa.202503341

Athanasios Kyritsis, Rafailia Aa Beta, Diana Scutelnic, Vasiliki Stravokefalou, Valerio Del Vescovo, Zoi V Arsenopoulou, Konstantinos Papikinos, Margherita Grasso, Francesca Fontana, Paraskevi Moutopoulou, Alexandros Tsiporis, Martina Samiotaki, George Panayotou, Michela A Denti, Nikolaos Aa Balatsos

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引用次数: 0

Abstract

The majority of pri-miRNAs acquire a 5' cap and 3' poly(A) tail. Mature miRNAs recruit deadenylases that shorten poly(Α) tails triggering target mRNA degradation. Poly(A)-specific ribonuclease (PARN) is a deadenylase that also mediates late steps of noncoding RNA maturation. Herein, we show that PARN affects the expression of a subset of miRNAs in NCI-H520 cells of lung cancer origin, including miR-29a and miR-1207, which are also predicted to target PARN mRNA. PARN associates with pri-miR-29a and pri-miR-1207 regulating their poly(A) lengths. Conversely, miR-29a-3p and miR-1207-5p bind the 3' UTR of PARN mRNA and regulate its expression. Cleavage and polyadenylation specificity factor 6 (CPSF6) recruits PARN to pri-miRNAs and together they affect primary and mature miR-29a-3p levels. Modulation of PARN, miR-29a-3p, or miR-1207-5p expression affects cell migration. We present a model to describe the dynamic relation between PARN and miR-29a and discuss its biological significance.

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聚(A)特异性核糖核酸酶（PARN）与同源microrna之间的相互反馈调节。

大多数的pri- mirna获得一个5‘帽和3’ poly(a) tail。成熟的mirna招募缩短聚（Α）尾部的死酶，从而触发靶mRNA降解。聚(A)特异性核糖核酸酶（PARN）是一种deadenylase，也介导非编码RNA成熟的后期步骤。本文中，我们发现PARN会影响肺癌起源的NCI-H520细胞中一部分mirna的表达，包括miR-29a和miR-1207，它们也被预测会靶向PARN mRNA。PARN与调节poly(A)长度的pri-miR-29a和pri-miR-1207相关。相反，miR-29a-3p和miR-1207-5p结合PARN mRNA的3' UTR并调节其表达。切割和聚腺苷化特异性因子6 （CPSF6）将PARN招募到pri- mirna，并共同影响初级和成熟的miR-29a-3p水平。PARN、miR-29a-3p或miR-1207-5p表达的调节影响细胞迁移。我们提出了一个模型来描述PARN与miR-29a之间的动态关系，并讨论其生物学意义。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Life Science Alliance Agricultural and Biological Sciences-Plant Science

CiteScore

5.80

自引率

2.30%

发文量

241

审稿时长

10 weeks

期刊介绍： Life Science Alliance is a global, open-access, editorially independent, and peer-reviewed journal launched by an alliance of EMBO Press, Rockefeller University Press, and Cold Spring Harbor Laboratory Press. Life Science Alliance is committed to rapid, fair, and transparent publication of valuable research from across all areas in the life sciences.