Impact of fluoxetine on innate immunity and melanoma metastasis in mice

IF 2.5 4区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL

Advances in medical sciences Pub Date : 2026-03-01 Epub Date: 2026-03-12 DOI:10.1016/j.advms.2026.03.003

Beata Grygier , Elzbieta Kolaczkowska , Monika Leśkiewicz , Katarzyna Curzytek , Michael Maes , Marta Kubera

{"title":"Impact of fluoxetine on innate immunity and melanoma metastasis in mice","authors":"Beata Grygier , Elzbieta Kolaczkowska , Monika Leśkiewicz , Katarzyna Curzytek , Michael Maes , Marta Kubera","doi":"10.1016/j.advms.2026.03.003","DOIUrl":null,"url":null,"abstract":"<div><h3>Purpose</h3><div>Fluoxetine, a selective serotonin reuptake inhibitor, is widely used for the treatment of depressive symptoms in cancer patients. However, there is conflicting evidence regarding its effects on immunity and cancer. The present study aimed to clarify the effect of fluoxetine administration before or after the initiation of B16F10 melanoma lung metastasis formation on cancer development.</div></div><div><h3>Methods</h3><div>In the present study, we used an experimental tumoral model of lung metastasis formation by melanoma B16F10 cells in C57BL/6J mice. Fluoxetine was administered 14 days before or 14 days after tumor cell injection. We assessed the mice's behavior in the forced swimming test, the formation of lung metastases, the proliferative and metabolic activity of splenocytes, and the activity of metalloproteinases.</div></div><div><h3>Results</h3><div>Results showed that repeated daily fluoxetine administration (10 mg/kg) after injection of tumor cells: 1) decreased the number of metastasis in the lungs; 2) decreased immobility time in forced swimming test; 3) increased proliferative and metabolic activity of splenocytes, 4) decreased ability of splenocytes to produce metalloproteinase 9 (MMP-9) whereas fluoxetine administration before injection of tumor cells induced opposite effect.</div></div><div><h3>Conclusion</h3><div>Obtained data confirm the inhibitory effect of fluoxetine on melanoma growth, but only when this antidepressant is given to animals that already struggle with melanoma development. Fluoxetine administration before cancer development accelerates its progress and induces unbeneficial changes in immunity and metalloproteinase levels in comparison to saline-pretreated mice.</div></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"71 1","pages":"Pages 95-105"},"PeriodicalIF":2.5000,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Advances in medical sciences","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S189611262600012X","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2026/3/12 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"MEDICINE, RESEARCH & EXPERIMENTAL","Score":null,"Total":0}

引用次数: 0

Abstract

Purpose

Fluoxetine, a selective serotonin reuptake inhibitor, is widely used for the treatment of depressive symptoms in cancer patients. However, there is conflicting evidence regarding its effects on immunity and cancer. The present study aimed to clarify the effect of fluoxetine administration before or after the initiation of B16F10 melanoma lung metastasis formation on cancer development.

Methods

In the present study, we used an experimental tumoral model of lung metastasis formation by melanoma B16F10 cells in C57BL/6J mice. Fluoxetine was administered 14 days before or 14 days after tumor cell injection. We assessed the mice's behavior in the forced swimming test, the formation of lung metastases, the proliferative and metabolic activity of splenocytes, and the activity of metalloproteinases.

Results

Results showed that repeated daily fluoxetine administration (10 mg/kg) after injection of tumor cells: 1) decreased the number of metastasis in the lungs; 2) decreased immobility time in forced swimming test; 3) increased proliferative and metabolic activity of splenocytes, 4) decreased ability of splenocytes to produce metalloproteinase 9 (MMP-9) whereas fluoxetine administration before injection of tumor cells induced opposite effect.

Conclusion

Obtained data confirm the inhibitory effect of fluoxetine on melanoma growth, but only when this antidepressant is given to animals that already struggle with melanoma development. Fluoxetine administration before cancer development accelerates its progress and induces unbeneficial changes in immunity and metalloproteinase levels in comparison to saline-pretreated mice.

查看原文本刊更多论文

氟西汀对小鼠先天免疫和黑色素瘤转移的影响。

目的：氟西汀是一种选择性5 -羟色胺再摄取抑制剂，广泛用于治疗癌症患者的抑郁症状。然而，关于它对免疫和癌症的影响，有相互矛盾的证据。本研究旨在阐明在B16F10黑色素瘤肺转移形成开始之前或之后给予氟西汀对癌症发展的影响。方法：本研究采用C57BL/6J小鼠黑素瘤B16F10细胞形成肺转移的实验肿瘤模型。氟西汀在肿瘤细胞注射前14天或后14天给药。观察小鼠在强迫游泳实验中的行为、肺转移灶的形成、脾细胞的增殖和代谢活性以及金属蛋白酶的活性。结果：结果显示，肿瘤细胞注射后每日重复给药氟西汀（10 mg/kg）： 1)减少肺转移灶数量；2)强迫游泳试验中静止时间缩短；3)增加了肿瘤细胞的增殖和代谢活性，4)降低了肿瘤细胞产生金属蛋白酶9 （MMP-9）的能力，而注射肿瘤细胞前给药氟西汀则产生相反的作用。结论：获得的数据证实氟西汀对黑色素瘤生长的抑制作用，但只有当这种抗抑郁药给予已经与黑色素瘤发展作斗争的动物时。与盐水预处理的小鼠相比，在癌症发生前服用氟西汀会加速其进展，并诱导免疫和金属蛋白酶水平的不利变化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Advances in medical sciences 医学-医学：研究与实验

CiteScore

5.00

自引率

0.00%

发文量

审稿时长

25 days

期刊介绍： Advances in Medical Sciences is an international, peer-reviewed journal that welcomes original research articles and reviews on current advances in life sciences, preclinical and clinical medicine, and related disciplines. The Journal’s primary aim is to make every effort to contribute to progress in medical sciences. The strive is to bridge laboratory and clinical settings with cutting edge research findings and new developments. Advances in Medical Sciences publishes articles which bring novel insights into diagnostic and molecular imaging, offering essential prior knowledge for diagnosis and treatment indispensable in all areas of medical sciences. It also publishes articles on pathological sciences giving foundation knowledge on the overall study of human diseases. Through its publications Advances in Medical Sciences also stresses the importance of pharmaceutical sciences as a rapidly and ever expanding area of research on drug design, development, action and evaluation contributing significantly to a variety of scientific disciplines. The journal welcomes submissions from the following disciplines: General and internal medicine, Cancer research, Genetics, Endocrinology, Gastroenterology, Cardiology and Cardiovascular Medicine, Immunology and Allergy, Pathology and Forensic Medicine, Cell and molecular Biology, Haematology, Biochemistry, Clinical and Experimental Pathology.